Anti-Human FAP & IL-2R Reference Antibody (Simlukafusp Alfa, RUO) (HC440026)

Name: Anti-Human FAP & IL-2R Reference Antibody (Simlukafusp Alfa, RUO)
Brand: abinScience
SKU: HC440026
Price: 400 USD
Availability: InStock

데이터시트 제품 공유 정보 복사

가격(USD):

사양:

100ug
1mg
5mg
10mg
25mg
50mg
100mg

수량:

문의하기

개요
이미지
참고 문헌
데이터시트
SDS

개요

카탈로그 번호	HC440026
설명	Simlukafusp alfa (HC440026) is a research-grade recombinant antibody targeting Surface-expressed protease. Produced in mammalian cells with native-like glycosylation. Highlights ●Research Grade — For PK/PD studies, assay development, and ADA research. ●Native Glycosylation — Mammalian expression ensures native-like patterns.
종 반응성	Human
응용 분야	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
숙주 종	Human
클론성	Monoclonal
이소타입	IgG1-kappa
발현 시스템	Mammalian cells
테스트된 응용 분야	WB, FCM
타겟	Surface-expressed protease, Gelatine degradation protease FAP, Integral membrane serine protease, Dipeptidyl peptidase FAP, Seprase, FAP, Post-proline cleaving enzyme, APCE, SIMP, FAPalpha, 170 kDa melanoma membrane-bound gelatinase, Fibroblast activation protein alpha, Serine integral membrane protease, Prolyl endopeptidase FAP
내독소 수준	< 10 EU/mg
순도	>95% purity as determined by SDS-PAGE.
정제	Protein A/G purified from cell culture supernatant.
등록번호	Q12884
형태	Liquid
저장 완충액	0.01M PBS pH 7.4 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.
안정성 및 저장	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
배경	Prolyl endopeptidase FAP (FAPalpha & IL2V) is a ~87 kDa protein. Cell surface glycoprotein serine protease that participates in extracellular matrix degradation and involved in many cellular processes including tissue remodeling, fibrosis, wound healing, inflammation and tumor growth. Both plasma membrane and soluble forms exhibit post-proline cleaving endopeptidase activity, with a marked preference for Ala/Ser-Gly-Pro-Ser/Asn/Ala consensus sequences, on substrate such as alpha-2-antiplasmin SERPINF2 and SPRY2. Degrade also gelatin, heat-denatured type I collagen, but not native collagen type I and IV, vitronectin, tenascin, laminin, fibronectin, fibrin or casein. Also has dipeptidyl peptidase activity, exhibiting the ability to hydrolyze the prolyl bond two residues from the N-terminus of synthetic dipeptide substrates provided that the penultimate residue is proline, with a preference for Ala-Pro, Ile-Pro, Gly-Pro, Arg-Pro and Pro-Pro. Natural neuropeptide hormones for dipeptidyl peptidase are the neuropeptide Y (NPY), peptide YY (PYY), substance P (TAC1) and brain natriuretic peptide 32 (NPPB). 1. Lee, KN. et al. (2004) Blood 103, 3783-8. PMID: 14751930 2. Lee, KN. et al. (2006) Blood 107, 1397-404. PMID: 16223769 3. Edosada, CY. et al. (2006) The Journal of biological chemistry 281, 7437-44. PMID: 16410248 4. Edosada, CY. et al. (2006) FEBS letters 580, 1581-6. PMID: 16480718 5. Meadows, SA. et al. (2007) Biochemistry 46, 4598-605. PMID: 17381073 6. Aggarwal, S. et al. (2008) Biochemistry 47, 1076-86. PMID: 18095711 7. Huang, CH. et al. (2011) Journal of biochemistry 149, 685-92. PMID: 21288888 8. Keane, FM. et al. (2013) FEBS open bio 4, 43-54. PMID: 24371721 9. Levy, MT. et al. (1999) Hepatology (Baltimore, Md.) 29, 1768-78. PMID: 10347120 10. Mueller, SC. et al. (1999) The Journal of biological chemistry 274, 24947-52. PMID: 10455171
참고사항	For research use only. Not suitable for clinical or therapeutic use.

이미지

SEC-HPLC

SEC-HPLC detection for Research Grade Simlukafusp alfa.
Bioactivity

Detects FAP/FAPalpha in indirect ELISAs.
SDS-PAGE

SDS-PAGE for Research Grade Simlukafusp alfa.
Flow CytoMetry

Flow-cytometry using FITC anti-human FAP&IL2V antibody. U87 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human FAP&IL2V monoclonal antibody (Catalog: HC440026, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using anti-human FAP antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human FAP monoclonal antibody (Catalog: HC440026) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Western blot

Western blot analysis was performed using anti-FAP monoclonal antibody at 1μg/ml on various samples.
Lane 1: recombinant human FAP (Catalog No: HC440011), 50ng
Lane 2: recombinant human FAP (Catalog No: HC440011), 30ng
Lane 3: recombinant human FAP (Catalog No: HC440011), 20ng