Anti-Human IL-4 Reference Antibody (Vak-694, RUO) (HY457026)

Name: Anti-Human IL-4 Reference Antibody (Vak-694, RUO)
Brand: abinScience
SKU: HY457026
Price: 326 USD
Availability: InStock

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사양:

100ug
1mg
5mg
10mg
25mg
50mg
100mg

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개요
이미지
참고 문헌
데이터시트
SDS

개요

카탈로그 번호

HY457026

설명

Anti-Human IL-4 Reference Antibody (Vak-694, RUO) (HY457026) is a human monoclonal antibody detecting IL-4. Expressed in Mammalian cells.

종 반응성

Human

응용 분야

ELISA, Bioactivity: FCM, Functional assay, Research in vivo, WB

숙주 종

Human

클론성

Monoclonal

이소타입

IgG1

생물학적 활성

VAK-694 binds to IL-4, reduces allergen-specific IL-4 producing cell numbers [1]. VAK-694 exerts immunomodulatory effects on TH2 memory cells [2].

발현 시스템

Mammalian cells

성질

Recombinant

테스트된 응용 분야

타겟

Binetrakin, B-cell stimulatory factor 1, Pitrakinra, Lymphocyte stimulatory factor 1, IL-4, BSF-1, IL4, Interleukin-4

내독소 수준

< 10 EU/mg as determined by LAL method.

순도

>95% as determined by SDS-PAGE.

정제

Protein A/G purified from cell culture supernatant.

등록번호

P05112

형태

Liquid

저장 완충액

0.01M PBS pH 7.4.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

대체 이름

VAK-694, NVP-VAK-694

배경

Interleukin-4 (IL4) is a ~17 kDa protein. Cytokine secreted primarily by mast cells, T-cells, eosinophils, and basophils that plays a role in regulating antibody production, hematopoiesis and inflammation, and the development of effector T-cell responses. Induces the expression of class II MHC molecules on resting B-cells. Enhances both secretion and cell surface expression of IgE and IgG1. Also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages.

1. Carr, C. et al. (1991) Biochemistry 30, 1515-23. PMID: 1993171

2. Yokota, T. et al. (1986) Proceedings of the National Academy of Sciences of the United States of America 83, 5894-8. PMID: 3016727

3. Delespesse, G. et al. (1989) Journal of immunology (Baltimore, Md. : 1950) 142, 134-8. PMID: 2521231

4. Hage, T. et al. (1999) Cell 97, 271-81. PMID: 10219247

5. Hülsmeyer, M. et al. (2001) Acta crystallographica. Section D, Biological crystallography 57, 1334-6. PMID: 11526337

6. LaPorte, SL. et al. (2008) Cell 132, 259-72. PMID: 18243101

7. Malabarba, MG. et al. (1995) The Journal of biological chemistry 270, 9630-7. PMID: 7721895

참고 문헌

1. Koneczny I, et al. IgG4 Autoantibodies in Organ-Specific Autoimmunopathies: Reviewing Class Switching, Antibody-Producing Cells, and Specific Immunotherapies. Front Immunol. 2022;13:834342. Published 2022 Mar 24. [HY457026]
2. Pfaar O, et al. Anti-IgE: A treatment option in allergic rhinitis?. Allergol Select. 2021;5:119-127. Published 2021 Feb 24. [HY457026]

참고사항

For research use only. Not suitable for clinical or therapeutic use.

Isotype Control

Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)

이미지

SDS-PAGE

SDS-PAGE for Research Grade VAK-694.
Bioactivity

Detects recombinant IL4 (Catalog No: HY457021) in indirect ELISA.
Western blot

Western blot analysis was performed using anti-IL4 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human IL4 (Catalog No: HY457021)
Lane 2: Negative control

참고 문헌

데이터시트

Anti-Human IL-4 Reference Antibody (Vak-694, RUO).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-Human IL-4 Reference Antibody (Vak-694, RUO) (HY457026)

개요

이미지

참고 문헌

데이터시트

SDS