Newcastle Disease Virus (NDV) Research Reagents — Antibodies & Recombinant Proteins

Hemagglutinin-Neuraminidase (HN)
HN is a multifunctional surface glycoprotein that binds sialic acid receptors (receptor attachment), cleaves sialic acid (neuraminidase activity to prevent self-aggregation and facilitate release), and triggers F protein activation for membrane fusion. HN is the primary target for NDV serological surveillance (HI assay) and a key component of NDV-vectored vaccine characterization. Available:
• HN Recombinant Protein C-Fc (VK544011) — Fc-tagged for Protein A capture in ELISA and SPR binding studies
• HN Recombinant Protein N-His (VK544012) — His-tagged for direct plate coating and pull-down assays
• Anti-HN Polyclonal Antibody (VK544014) — for WB, ELISA, and IHC detection of HN in infected tissues and vaccine preparations

Nucleoprotein (NP)
NP encapsidates the viral RNA genome and is the most abundant structural protein in NDV-infected cells. NP is the standard target for NDV diagnostic RT-PCR and antigen-capture assays, and a key QC marker for NDV-vectored vaccine production (monitoring vector replication). Available:
• NP Recombinant Protein N-His (VK544032) — for ELISA coating, antibody screening, and as a reference standard
• Anti-NP Polyclonal Antibody (VK544034) — for WB detection and IHC in tissue sections

Which HN protein format is best for serology (HI assay replacement)?
HN N-His (VK544012) is recommended for direct ELISA plate coating as an alternative to traditional hemagglutination inhibition (HI) assays. For sandwich or capture-based formats, HN C-Fc (VK544011) can be captured on Protein A-coated plates for oriented antigen presentation.

Do you have NDV F protein reagents?
F protein (fusion) is the primary virulence determinant — velogenic strains have a polybasic F cleavage site. F protein reagents are currently in development. Contact us for availability or to request custom F protein variants (specific cleavage site mutants, prefusion-stabilized F).

Can these reagents be used for oncolytic NDV research?
Yes. Our HN and NP reagents can be used to characterize oncolytic NDV preparations: HN protein and antibody for quantifying surface HN expression on oncolytic NDV virions, and NP antibody for monitoring NDV replication in tumor cell lysates by WB or IHC. For recombinant NDV-vectored vaccines (e.g., NDV-HXP-S expressing SARS-CoV-2 spike), use our NDV reagents alongside target-specific reagents from our SARS-CoV-2 page.

Explore reagents for related viruses:

NDV ✓ IBV (Avian coronavirus) Influenza A (AIV) ASFV CDV Nipah / Hendra