Avian Infectious Bronchitis Virus (IBV) Research Reagents — Antibodies, Recombinant Proteins & ELISA Kit

The S1 subunit determines IBV serotype and contains the major neutralizing epitopes. S1 is cleaved from S2 by furin-like proteases and is the primary component of IBV vaccines and diagnostic typing assays. The NTD mediates receptor binding, while the CTD contains serotype-specific hypervariable regions. abinScience provides S1 proteins in both domain-specific and full-subunit formats:

S1 N-Terminal Domain (NTD)
• S1-NTD C-Fc (VK710021) — Fc-tagged for Protein A capture in binding and neutralization assays
• S1-NTD N-His (VK710022) — His-tagged for direct plate coating
• S1-NTD C-His (VK710031)
• Anti-S1-NTD polyclonal antibody (VK710024)

S1 C-Terminal Domain (CTD)
• S1-CTD C-Fc (VK710011)
• S1-CTD N-His (VK710032)
• S1-CTD C-His (VK710041)
• Anti-S1-CTD polyclonal antibody (VK710034)

Full S1 Subunit
• S1 full-length C-His (VK710012)
• Anti-S1 polyclonal antibody (VK710014)

3CL Protease (NSP5)
The 3CL protease (chymotrypsin-like main protease) cleaves the replicase polyprotein and is a primary target for broad-spectrum coronavirus antiviral development. IBV 3CL is structurally homologous to SARS-CoV-2 Mpro, enabling cross-application of protease inhibitor screening platforms. Available: recombinant 3CL protein N-His (VK024022) and anti-3CL polyclonal antibody (VK024024).

NSP3 (Papain-Like Protease & X/Macro Domain)
NSP3 is the largest non-structural protein, containing the papain-like protease (PLpro), X/macro domain (ADP-ribose-1″-phosphatase), and multiple functional domains. The X domain removes ADP-ribosylation marks from host proteins to suppress innate immunity. Available: NSP3 full-length N-His (VK024012), NSP3 X domain N-His (VK024052), anti-NSP3 pAb (VK024014), and anti-NSP3 X domain pAb (VK024054).

NSP2 & NSP9 (Replicase Components)
• NSP2: recombinant protein N-His (VK024032) and anti-NSP2 pAb (VK024034)
• NSP9 (ssRNA-binding protein, RdRp cofactor): recombinant protein N-GST&C-His (VK024042) and anti-NSP9 pAb (VK024044)

Chickens IBV Antibody ELISA Kit (VK710018)
Ready-to-use indirect ELISA kit for detection of anti-IBV antibodies in chicken serum. Applications include post-vaccination immune monitoring, flock seroprevalence surveys, and confirmation of IB diagnosis alongside RT-PCR.

Which S1 protein format is best for serotype-specific ELISA?
S1-CTD contains the serotype-specific hypervariable regions (HVR1, HVR2, HVR3) — use S1-CTD C-Fc (VK710011) for serotype discrimination. S1-NTD is more conserved and better suited for broadly reactive detection across serotypes. Full-length S1 C-His (VK710012) captures both domain-specific and conformational epitopes.

Can I use IBV 3CL protease for broad-spectrum coronavirus antiviral screening?
Yes. IBV 3CL protease shares structural homology with SARS-CoV-2 Mpro and other coronavirus main proteases. Our 3CL protein (VK024022) is suitable for in vitro protease cleavage assays and high-throughput inhibitor screening. Cross-reference with our SARS-CoV-2 NSP panel for comparative studies across coronavirus species.

What is the NSP3 X/macro domain and why is it relevant?
The X/macro domain is a conserved coronavirus enzyme that removes ADP-ribosylation marks deposited by host PARP enzymes as an innate immune defense. Inhibiting the macro domain restores host antiviral signaling. Our X domain protein (VK024052) is an ideal substrate for macro domain inhibitor screening — a hot target in pan-coronavirus antiviral development.

Explore reagents for related coronaviruses and avian viruses:

IBV ✓ SARS-CoV-2 SL-CoVs / Seasonal HCoVs MHV PEDV PDCoV NDV Influenza (AIV)