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Anti-IBV nsp3 Polyclonal Antibody (VK024014)

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Overview
Catalog No.VK024014
Description
Anti-IBV nsp3 Polyclonal Antibody (VK024014) is a rabbit polyclonal antibody detecting nsp3 in ELISA, IHC, WB.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityAvian infectious bronchitis virus (IBV)
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant IBV Replicase polyprotein 1ab (Thr1174-Gln1483).
Target Papain-like protease, PL-PRO, EC:3.4.19.12, EC:3.4.22.-, Non-structural protein 3, nsp3, p195
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession P0C6Y1, P0C6Y2, P0C6V3, P0C6V4, P0C6Y3, P0C6V5
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
WB 1:500-1:2000
IHC 1:50-1:500
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Replicase polyprotein 1ab (nsp3) is a ~744 kDa protein. Multifunctional protein involved in the transcription and replication of viral RNAs. Contains the proteinases responsible for the cleavages of the polyprotein.

Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-IBV nsp3 Polyclonal Antibody

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