Nipah Virus (NiV) & Hendra Virus (HeV) Research Reagents — Antibodies, Recombinant Proteins & ELISA Kits

Nipah virus (NiV) and Hendra virus (HeV) are enveloped, non-segmented, negative-sense RNA paramyxoviruses of the genus Henipavirus (~18.2 kb genome). NiV was first identified in 1999 following a large encephalitis outbreak in Malaysia/Singapore (case fatality 40–75%), while HeV was identified in 1994 in Australia (case fatality ~57%). Both are bat-borne BSL-4 pathogens on the WHO R&D Blueprint priority pathogen list. Henipaviruses encode six structural proteins: the attachment glycoprotein G (binds ephrin-B2/B3 receptors), fusion protein F (class I fusogen), matrix M, nucleoprotein N, phosphoprotein P (plus accessory V, W, C proteins from the P gene), and RNA polymerase L. The G protein is the primary target for neutralizing antibodies and vaccines — Nipah G is the immunogen in the leading NiV vaccine candidates currently in clinical trials.

Nipah/Hendra virus (Henipavirus) structure diagram showing G protein (attachment), F protein (fusion), M protein (matrix), and ribonucleoprotein complex

Henipavirus virion structure. G protein mediates attachment to ephrin-B2/B3; F protein drives membrane fusion; M protein scaffolds virion assembly; RNP complex encapsidates the ~18 kb (−)ssRNA genome.

Research Use OnlyNot for diagnostic or therapeutic use.

abinScience offers one of the most comprehensive Nipah & Hendra virus reagent portfolios available — spanning both viruses with cross-reactive and species-specific antibodies, including biosimilar-grade reference antibodies, InVivoMAb neutralizing antibodies (anti-G, anti-F, anti-prefusion), recombinant proteins covering G, F, N, M, and P, nanobodies, ELISA kits, and antibody pair sets. All products are recombinant — no BSL-4 required. Manufactured by AtaGenix Laboratories (ISO 9001 & ISO 13485). Contact us for antibody pairs, epitope panels, and bulk orders.

G Protein (Attachment Glycoprotein) — Primary Vaccine & Therapeutic Target

The G protein is the receptor-binding protein that engages ephrin-B2 (widely expressed) and ephrin-B3 (predominantly neural) on host cells. G is the dominant neutralizing antigen and the immunogen in all leading NiV vaccine candidates (Oxford/Janssen ChAdOx1-NiV, PHV02/mRNA, IAVI subunit). Anti-G antibodies block receptor attachment and are the most potent neutralizers. abinScience provides the deepest anti-G antibody panel available:

Cross-Reactive NiV/HeV Anti-G Antibodies
• m102.3 (VK685013) — the best-characterized henipavirus bnAb, validated in African green monkey NiV challenge
• m102.4 (VK685023) — human IgG1 variant of m102.3, used under compassionate-use protocol
• HENV-26 (VK685033), HENV-32 (VK685043), HENV-103 (VK685053) — human mAbs from the Crowe panel with broadly neutralizing activity
• Monoclonal antibodies: 1A050 (VK685015), 1A206 (VK685025), 1A207 (VK685035)

InVivoMAb Anti-G (In Vivo Grade)
Low-endotoxin, azide-free antibodies for animal challenge studies: VK631010, VK631020, and additional format variants for NiV-specific and HeV-specific studies.

Recombinant G Proteins & ELISA Kits
• NiV G protein: C-Fc (VK631011), C-His (VK631021)
• HeV G protein: C-Fc (VK685011), C-His (VK685021)
• Anti-G polyclonal antibody (VK631014)
• Nanobodies: SAA1104 (VK631013) and SAA1249 (VK631023)

F Protein (Fusion) & Prefusion Antibodies

The F protein is a class I fusion glycoprotein cleaved into F1/F2 subunits. Anti-F antibodies block membrane fusion after G-mediated attachment and provide a complementary neutralization mechanism to anti-G. Prefusion-stabilized F is an emerging vaccine antigen target.

Anti-F Recombinant Antibodies (Cross-Reactive NiV/HeV)
• mAb66 (VK521013) — broadly neutralizing anti-F antibody
• 5B3 (VK431013), 12B2 (VK431023), 1F5 (VK431033) — anti-F clones recognizing distinct epitopes

InVivoMAb Anti-Prefusion NiV Antibodies
Prefusion-conformation-specific antibodies for in vivo studies: 4H3 (VK521010), 1H8 (VK521020), 1A9 (VK521030), and 2B12 (VK521050).

Recombinant F Proteins
• NiV F protein: VK521011, VK521012
• HeV F protein: VK431011, VK431012
• Anti-F polyclonal antibody (VK521014)

N, M, P & Internal Proteins — Diagnostics & Replication

Nucleoprotein (N)
NP is the most abundant protein in infected cells and the primary target for diagnostic antigen-capture assays and serological IgG/IgM detection. Available: NiV NP recombinant proteins (N-His VK432012, N-His variant VK432022), HeV NP (N-His VK432032), anti-NP pAb (VK432014), and NiV NP Antibody Pair Set (VK432908) for sandwich ELISA development.

Matrix (M) & Phosphoprotein (P)
• M protein: NiV (VK499012, VK408012) and HeV (VK499022); polyclonal antibodies VK499014 and VK408014
• Phosphoprotein: NiV P protein N-His (VK078012)

Frequently Asked Questions

Which antibodies cross-react with both NiV and HeV?
Most of our anti-G and anti-F antibodies are cross-reactive by design — NiV and HeV G proteins share ~83% amino acid identity, and F proteins share ~89%. Key cross-reactive clones: m102.3 and m102.4 (anti-G), and mAb66 and 5B3 (anti-F). For species-specific detection, use monoclonal antibodies paired with NiV-specific or HeV-specific recombinant G/F proteins.

Which antibody combination provides the broadest protection?
Combining anti-G (blocks attachment) + anti-F (blocks fusion) provides complementary neutralization. A two-antibody cocktail of m102.3 (anti-G) + mAb66 (anti-F) covers both entry steps and is protective in animal models. Adding prefusion-specific antibodies (4H3, 1H8) may further enhance potency.

Can I work with these reagents at BSL-2?
Yes. All products are recombinant proteins and antibodies — no live or inactivated virus. Standard BSL-1/BSL-2 conditions are sufficient. Live NiV and HeV require BSL-4.

Do you have ELISA kits for NiV serology?
Yes. We offer ELISA kits and an NiV NP Antibody Pair Set (VK432908) for custom sandwich ELISA development. Individual NiV and HeV G/F/NP proteins in His/Fc formats are available for coating custom serology plates.

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