Anti-HeV/NiV F/Fusion glycoprotein F0 Antibody (5B3) (VK431013)

Datasheet Share product Copy info

Price(USD): $

Spec:

20ug
50ug
100ug
1mg

Number:

Overview
Images
References
Datasheet

Overview

Catalog No.

VK431013

Description

Anti-HeV/NiV F/Fusion glycoprotein F0 Antibody (5B3) [5B3] (VK431013) is a mouse monoclonal antibody detecting Fusion glycoprotein F0 in ELISA, FCM, Neutralization.

Highlights

●Multi-Application — Validated across multiple applications.
●Flow Cytometry — Validated for flow cytometry applications.

Species reactivity

Hendra virus (isolate Horse/Autralia/Hendra/1994) & Nipah virus

Applications

ELISA, FCM, Neutralization

Host species

Mouse

Isotype

IgG1, kappa

Clone ID

5B3

Clonality

Monoclonal

Target

F, Fusion glycoprotein F0, Protein F

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

O89342, Q9IH63

RRID

Anti-HeV/NiV F/Fusion glycoprotein F0 Antibody (5B3) (abinScience Cat# VK431013, RRID:AB_3725238)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Alternate Names

5B3, h5B3.1

Background

Fusion glycoprotein F0 is a ~59 kDa protein. Class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and plasma cell membrane fusion, the heptad repeat (HR) regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and plasma cell membranes. Directs fusion of viral and cellular membranes leading to delivery of the nucleocapsid into the cytoplasm.

Note

For research use only.

Images