Nipah virus/NiV M/Matrix Recombinant Protein (N-His) (VK408012)

Name: Nipah virus/NiV M/Matrix Recombinant Protein (N-His)
Brand: abinScience
SKU: VK408012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	VK408012
Description	_x005F_x000D_ _x005F_x000D_ _x005F_x000D_ _x005F_x000D_ _x005F_x000D_ _x005F_x000D_ Recombinant Nipah virus/NiV M/Matrix Protein, N-His (VK408012) expressed in E. coli, spanning Met1-Gly352. Purity: >90% by SDS-PAGE. _x005F_x000D_ _x005F_x000D_ _x005F_x000D_ Highlights _x005F_x000D_ _x005F_x000D_ _x005F_x000D_ ●His-Tagged — N-terminal 6×His tag for IMAC purification. _x005F_x000D_ ●E. coli Expression — High-yield, cost-effective production. _x005F_x000D_ ●High Purity — >90% purity verified by SDS-PAGE. _x005F_x000D_ _x005F_x000D_ _x005F_x000D_
Expression system	E. coli
Accession	Q9IK90
Protein length	Met1-Gly352
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Nipah virus
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	42.09 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Matrix protein, Protein M, M protein
Background	Matrix protein (Protein M) is a ~39 kDa protein. Plays a crucial role in virion assembly and budding. Forms a shell at the inner face of the plasma membrane. Transits through the host nucleus before gaining the functional ability to localize and bud from the plasma membrane. Mediates together with fusion protein the incorporation of the glycoprotein to the viral particles. Also participates in the inhibition of the host interferon type I antiviral response by interacting with and thereby inhibiting host TRIM6. _x005F_x000D_ _x005F_x000D_ 1. Ciancanelli, MJ. et al. (2006) Journal of virology 80, 12070-8. PMID: 17005661 _x005F_x000D_ _x005F_x000D_ 2. Patch, JR. et al. (2007) Virology journal 4, 1. PMID: 17204159 _x005F_x000D_ _x005F_x000D_ 3. Patch, JR. et al. (2008) Virology journal 5, 137. PMID: 19000317 _x005F_x000D_ _x005F_x000D_ 4. Wang, YE. et al. (2010) PLoS pathogens 6, e1001186. PMID: 21085610 _x005F_x000D_ _x005F_x000D_ 5. Johnston, GP. et al. (2017) Journal of virology 91. PMID: 28250132 _x005F_x000D_ _x005F_x000D_ 6. Bharaj, P. et al. (2016) PLoS pathogens 12, e1005880. PMID: 27622505 _x005F_x000D_
Note	For research use only.