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Recombinant Nipah virus/NiV Protein N/Nucleoprotein Protein, N-His (VK432012)

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Recombinant Nipah virus/NiV Protein N/Nucleoprotein Protein, N-His

Recombinant Nipah virus/NiV Protein N/Nucleoprotein Protein, N-His

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Datasheet

Overview

Catalog No.	VK432012
Description	Recombinant Nipah virus/NiV Protein N/Nucleoprotein Protein, N-His (VK432012) expressed in E. coli, spanning Thr32-Val532. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q9IK92
Protein length	Thr32-Val532
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Nipah virus (NiV)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	57.14 kDa
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Nucleoprotein, Protein N, Nucleocapsid protein, N
Background	Nucleoprotein (Protein N) is a ~58 kDa protein. Forms the helical nucleocapsid (NC) in a ratio of 1 N per 6 ribonucleotides, protecting the genome from nucleases. The nucleocapsid (NC) has a helical structure with either 13.4 N per turn, approximately 20 nm in diameter, with a hollow central cavity of 56 nm in diameter. The encapsidated genomic RNA serves as template for transcription and replication; encapsidation by N is coupled to RNA synthesis. Forms the encapsidation complex with the phosphoprotein protein P. Before encapsidation, the newly synthesized free N protein, so-called N0, is chaperoned by P. 1. Ker, DS. et al. (2021) PLoS pathogens 17, e1009740. PMID: 34270629 2. Yabukarski, F. et al. (2014) Nature structural & molecular biology 21, 754-9. PMID: 25108352
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Nipah virus/NiV Protein N/Nucleoprotein Protein, N-His

References

VK432012-SDS-PAGE-1.jpg

Datasheet

Recombinant Nipah virus/NiV Protein N/Nucleoprotein Protein, N-His.pdf

Formula

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Recombinant Nipah virus/NiV Protein N/Nucleoprotein Protein, N-His