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Anti-NDV HN/Hemagglutinin-neuraminidase Polyclonal Antibody (VK544014)

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Overview
Catalog No.VK544014
Description
Anti-NDV HN/Hemagglutinin-neuraminidase Polyclonal Antibody (VK544014) is a rabbit polyclonal antibody detecting NDV HN/Hemagglutinin-neuraminidase in ELISA, IHC, WB.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityNewcastle disease virus (NDV) (Avian orthoavulavirus 1)
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant NDV HN/Hemagglutinin-neuraminidase (Pro126-Ile571).
Target HN glycoprotein, HN
Endotoxin level Please contact with the lab for this information.
Purity >95% as determined by SDS-PAGE.
Purification Purified by antigen affinity column.
Accession AAF06665.1
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Hemagglutinin-neuraminidase (HN) is a ~64 kDa protein. Attaches the virus to alpha-2,3-linked sialic acid-containing cell receptors and thereby initiating infection. Binding of HN protein to the receptor induces a conformational change that allows the F protein to trigger virion/cell membranes fusion. Binds to the glycan motifs sialyl Lewis (SLe) and GM2 ganglioside (GM2-glycan).

Note For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-NDV HN/Hemagglutinin-neuraminidase Polyclonal Antibody

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