中文
English
한국어
日本語
Español
Français
Русский
| 카탈로그 번호 | HB644013 |
|---|---|
| 설명 |
Anti-Human L-Selectin/CD62L Recombinant Antibody (SRR0039) (HB644013) is a rabbit monoclonal antibody detecting L-Selectin/CD62L in IF, FCM. Suitable for Human.
Highlights
|
| 종 반응성 | Human |
| 응용 분야 | IF, FCM |
| 숙주 종 | Rabbit |
| 클론성 | Monoclonal |
| 이소타입 | IgG |
| 클론 ID | SRR0039 |
| 테스트된 응용 분야 | IF, FCM |
| 타겟 | CD62 antigen-like family member L, CD62L, L-selectin, LAM-1, LECAM1, LNHR, LYAM1, Leukocyte adhesion molecule 1, Leukocyte surface antigen Leu-8, Leukocyte-endothelial cell adhesion molecule 1, Lymph node homing receptor, SELL, TQ1, gp90-MEL |
| 내독소 수준 | < 10 EU/mg as determined by LAL method. |
| 순도 | >95% as determined by SDS-PAGE. |
| 정제 | Protein A/G purified from cell culture supernatant. |
| 등록번호 | P14151 |
| 형태 | Liquid |
| 저장 완충액 | 0.01M PBS pH 7.4. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 배경 | L-selectin (CD62L/SELL) is a ~42 kDa protein. Calcium-dependent lectin that mediates cell adhesion by binding to glycoproteins on neighboring cells. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia. 1. Bernimoulin, MP. et al. (2003) The Journal of biological chemistry 278, 37-47. PMID: 12403782 2. Mehta-D'souza, P. et al. (2017) The Journal of biological chemistry 292, 2510-2518. PMID: 28011641 3. Wedepohl, S. et al. (2017) Chembiochem : a European journal of chemical biology 18, 1338-1345. PMID: 28489325 |
| 참고사항 | For research use only. |
Jurkat cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting L-Selectin/CD62L (HB644013) at a concentration of 20 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) at a concentration of 6.5 ug/ml for 50 minutes at room temperature. The localization of L-Selectin/CD62L was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).
Flow-cytometry using anti-Human CD62L antibody. Loucy cells were stained with an irrelevant antibody (Blue Histogram) or an anti-Human CD62L monoclonal antibody (Catalog HB644013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Rabbit IgG H&L Polyclonal Antibody, FITC (abinScience:TF690414) and cells analysed on a NovoCyte Flow Cytometer.
문의하기
+86-027-65523339
중국 우한시 심둔사로 666번지 C동, 우한, 430206
