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| 카탈로그 번호 | HW729033 |
|---|---|
| 종 반응성 | Human |
| 응용 분야 | IF |
| 숙주 종 | Rabbit |
| 클론성 | Monoclonal |
| 이소타입 | IgG |
| 클론 ID | SRR0173 |
| 테스트된 응용 분야 | IF |
| 타겟 | EC:2.8.2.4, EST-1, Estrogen sulfotransferase, ST1E1, STE, SULT1E1, Sulfotransferase 1E1, Sulfotransferase, estrogen-preferring |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >95% as determined by SDS-PAGE. |
| 정제 | Protein A/G purified from cell culture supernatant. |
| 등록번호 | P49888 |
| 형태 | Liquid |
| 저장 완충액 | 0.01M PBS pH 7.4. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 참고사항 | For research use only |
HeLa cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting SULT1E1 (HW729033) at a concentration of 20 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) at a concentration of 6.5 ug/ml for 50 minutes at room temperature. The localization of SULT1E1 was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).
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