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| 카탈로그 번호 | HB617043 |
|---|---|
| 설명 |
Anti-Human B-Raf Recombinant Antibody (SRR0002) (HB617043) is a rabbit monoclonal antibody detecting B-Raf in IF, WB. Suitable for Human.
Highlights
|
| 종 반응성 | Human |
| 응용 분야 | IF, WB |
| 숙주 종 | Rabbit |
| 클론성 | Monoclonal |
| 이소타입 | IgG |
| 클론 ID | SRR0002 |
| 테스트된 응용 분야 | IF, WB |
| 타겟 | BRAF, BRAF1, EC:2.7.11.1, Proto-oncogene B-Raf, RAFB1, Serine/threonine-protein kinase B-raf, p94, v-Raf murine sarcoma viral oncogene homolog B1 |
| 내독소 수준 | < 10 EU/mg as determined by LAL method. |
| 순도 | >95% as determined by SDS-PAGE. |
| 정제 | Protein A/G purified from cell culture supernatant. |
| 등록번호 | P15056 |
| 형태 | Liquid |
| 저장 완충액 | 0.01M PBS pH 7.4. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 배경 | Serine/threonine-protein kinase B-raf (BRAF) is a ~84 kDa protein. Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus (Probable). Phosphorylates MAP2K1, and thereby activates the MAP kinase signal transduction pathway. Phosphorylates PFKFB2. May play a role in the postsynaptic responses of hippocampal neurons. 1. Brennan, DF. et al. (2011) Nature 472, 366-9. PMID: 21441910 2. Lavoie, H. et al. (2018) Nature 554, 549-553. PMID: 29433126 3. McGrail, K. et al. (2022) Nature communications 13, 7113. PMID: 36402789 4. Stephens, RM. et al. (1992) Molecular and cellular biology 12, 3733-42. PMID: 1508179 |
| 참고사항 | For research use only. |
Western blot analysis was performed using anti-B-Raf monoclonal antibody at 1 ug/mL on various samples.
Lane 1: K562 cell lysate
Lane 2: Jurkat cell lysate
Lane 3: HEK293T cell lysate
HeLa cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting B-Raf (HB617043) at a concentration of 20 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) at a concentration of 6.5 ug/ml for 50 minutes at room temperature. The localization of B-Raf was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).
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