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HEK293T Cell Line (HG001818)

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개요
카탈로그 번호HG001818
설명
HEK293T Cell Line (HG001818) is a parental HEK293T cell line. Widely used as a host cell for transient transfection, stable cell line generation, and recombinant protein production.
Highlights
  • Parental Cell Line — Unmodified HEK293T cells suitable as host cells or negative controls.
  • High Transfection Efficiency — Contains the SV40 large T antigen for enhanced episomal replication of plasmids carrying the SV40 origin.
  • Versatile Applications — Ideal for transient transfection, lentiviral packaging, stable cell line development, and recombinant protein expression.
응용 분야FCM
Human
숙주 종 HEK293T
특성 Growth Properties: Adherent; Morphology: Epithelial; Culture Medium: DMEM + 10% FBS + 1% Penicillin-Streptomycin; Culture Conditions: 37°C, 5% CO₂
배송 Dry ice
안정성 및 저장 Frozen in liquid nitrogen.
사양 1 vial contains at least 2×10^6 cells in 1 mL Freeze Medium
대체 이름293T, HEK-293T, HEK 293T
배경

HEK293T is a highly transfectable derivative of the human embryonic kidney 293 (HEK293) cell line that stably expresses the SV40 large T antigen. The presence of the T antigen allows episomal replication of transfected plasmids containing the SV40 origin of replication, leading to amplification of the plasmid and extended temporal expression of the desired gene products. HEK293T cells are widely used for transient protein expression, lentiviral and retroviral packaging, and as host cells for generating stable cell lines overexpressing specific target genes. They grow as an adherent monolayer and are readily maintained in standard culture conditions.

참고사항 For research use only
이미지
참고 문헌
데이터시트
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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