Anti-ACTN4 Polyclonal Antibody (HT028014)

Name: Anti-ACTN4 Polyclonal Antibody
Brand: abinScience
SKU: HT028014
Price: 96 USD
Availability: InStock

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개요
이미지
참고 문헌
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SDS

개요

카탈로그 번호

HT028014

설명

Anti-ACTN4 Polyclonal Antibody (HT028014) is a rabbit polyclonal antibody detecting ACTN4 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Chicken.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

종 반응성

Human, Mouse, Rat

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human ACTN4 (Tyr611-Leu911).

타겟

Non-muscle alpha-actinin 4, Alpha-actinin-4, ACTN4

내독소 수준

Please contact with the lab for this information.

정제

Purified by antigen affinity column.

등록번호

O43707

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Alpha-actinin-4 (ACTN4) is a ~104 kDa protein. F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein (Probable). Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation. Involved in tight junction assembly in epithelial cells probably through interaction with MICALL2.

1. Yan, Q. et al. (2005) Molecular biology of the cell 16, 2470-82. PMID: 15772161

2. Khurana, S. et al. (2012) The Journal of biological chemistry 287, 12027-35. PMID: 22351778

3. Gordón-Alonso, M. et al. (2012) Journal of immunology (Baltimore, Md. : 1950) 189, 689-700. PMID: 22689882

참고사항

For research use only.

이미지

Western blot

Western blot analysis was performed using anti-ACTN4 polyclonal antibody at ACTN4 on various samples.
Lane 1: Hela cell lysate
Lane 2: Jurkat cell lysate
Lane 3: 293T cell lysate
Lane 4: NIH3T3 cell lysate
Lane 5: A431 cell lysate
Lane 6: Mouse brain lysate
Lane 7: Mouse heart lysate
Lane 8: Mouse skeletal muscle lysate
Lane 9: Mouse kidney lysate
Lane 10: Rat brain lysate

참고 문헌

데이터시트

Anti-ACTN4 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-ACTN4 Polyclonal Antibody (HT028014)

개요

이미지

참고 문헌

데이터시트

SDS