Anti-ASFV g5Rp/D250R Polyclonal Antibody (VK490014)

Name: Anti-ASFV g5Rp/D250R Polyclonal Antibody
Brand: abinScience
SKU: VK490014
Price: 168 USD
Availability: InStock

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100ug

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개요
이미지
참고 문헌
데이터시트
SDS

개요

카탈로그 번호

VK490014

설명

Anti-ASFV g5Rp/D250R Polyclonal Antibody (VK490014) is a rabbit polyclonal antibody detecting D250R in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

종 반응성

African swine fever virus (ASFV)

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant ASFV g5Rp/D250R (Met1-His250).

타겟

mRNA-decapping protein g5R, g5Rp, 3.1.3.-, ASFV-DP, Diphosphoinositol polyphosphate phosphohydrolase, DIPP, 3.6.1.52, Ba71V-102, D250R

정제

Purified by antigen affinity column.

등록번호

P32092

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

mRNA-decapping protein g5R (g5Rp/D250R) is a ~29 kDa protein. Decapping enzyme required for the removal of the 5'-end m7GpppN cap tethered to viral and host mRNAs to allow their decay in cells. May therefore accelerate viral and cellular mRNA turnover to eliminate competing host mRNAs and allow stage-specific synthesis of viral proteins. Acceleration of the turnover of cellular transcripts may even promote the shutoff of host protein synthesis. In addition to the mRNA cap, g5R also efficiently hydrolyzes diphosphoinositol polyphosphates. Down-regulation of the level of PP-InsP5 (diphosphoinositol pentakisphosphate) may play a role in viral manipulation of the cellular secretory pathway, a step necessary for the formation of virions.

1. Parrish, S. et al. (2009) Virology 393, 177-82. PMID: 19695654

2. Quintas, A. et al. (2017) Journal of virology 91. PMID: 29021398

3. Cartwright, JL. et al. (2002) Journal of virology 76, 1415-21. PMID: 11773415

참고사항

For research use only.

이미지

Western blot

Lane 1: recombinant ASFV g5Rp/D250R (Catalog No: VK490012)

참고 문헌

데이터시트

Anti-ASFV g5Rp/D250R Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-ASFV g5Rp/D250R Polyclonal Antibody (VK490014)

개요

이미지

참고 문헌

데이터시트

SDS

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