Anti-ATIC Polyclonal Antibody (HD440014)

Name: Anti-ATIC Polyclonal Antibody
Brand: abinScience
SKU: HD440014
Price: 96 USD
Availability: InStock

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개요
이미지
참고 문헌
데이터시트
SDS

개요

카탈로그 번호

HD440014

설명

Anti-ATIC Polyclonal Antibody (HD440014) is a rabbit polyclonal antibody detecting ATIC in ELISA, IHC, WB. Suitable for Rat, Mouse, and Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

종 반응성

Human, Mouse, Rat

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human ATIC (Lys225-His592).

타겟

5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase, AICAR formyltransferase, AICAR transformylase, AICAR transformylase/inosine monophosphate cyclohydrolase, ATIC, Bifunctional purine biosynthesis protein ATIC, Bifunctional purine biosynthesis protein ATIC, N-terminally processed, EC:2.1.2.3, EC:3.5.4.10, IMP cyclohydrolase, IMP synthase, Inosine 5'-monophosphate cyclohydrolase, Inosinicase, PURH, Phosphoribosylaminoimidazolecarboxamide formyltransferase

내독소 수준

Please contact with the lab for this information.

정제

Purified by antigen affinity column.

등록번호

P31939

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

대체 이름

P31939

배경

Bifunctional purine biosynthesis protein ATIC is a ~64 kDa protein. Bifunctional enzyme that catalyzes the last two steps of purine biosynthesis. Acts as a transformylase that incorporates a formyl group to the AMP analog AICAR (5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide) to produce the intermediate formyl-AICAR (FAICAR). Can use both 10-formyldihydrofolate and 10-formyltetrahydrofolate as the formyl donor in this reaction. Also catalyzes the cyclization of FAICAR to inosine monophosphate (IMP). Is able to convert thio-AICAR to 6-mercaptopurine ribonucleotide, an inhibitor of purine biosynthesis used in the treatment of human leukemias.

1. Bulock, KG. et al. (2002) The Journal of biological chemistry 277, 22168-74. PMID: 11948179

2. Vergis, JM. et al. (2004) Biochemistry 43, 1184-92. PMID: 14756554

3. Wall, M. et al. (2000) Biochemistry 39, 11303-11. PMID: 10985775

4. Sugita, T. et al. (1997) Journal of biochemistry 122, 309-13. PMID: 9378707

5. Boutchueng-Djidjou, M. et al. (2015) Molecular & cellular proteomics : MCP 14, 1079-92. PMID: 25687571

참고사항

For research use only

이미지

Western blot

Western blot analysis was performed using anti-ATIC polyclonal antibody at 1ug/mL on various samples.
Lane 1: 293T cell lysate
Lane 2: Hela cell lysate
Lane 3: HepG2 cell lysate
Lane 4: Jurkat cell lysate
Lane 5: HCT116 cell lysate
Lane 6: A549 cell lysate
Lane 7: MCF-7 cell lysate
Lane 8: HT-29 cell lysate
Lane 9: K562 cell lysate
Lane 10: NIH3T3 cell lysate
Lane11: PC-12 cell lysate

참고 문헌

데이터시트

Anti-ATIC Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-ATIC Polyclonal Antibody (HD440014)

개요

이미지

참고 문헌

데이터시트

SDS