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| 카탈로그 번호 | HB617014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 설명 |
Anti-BRAF Polyclonal Antibody (HB617014) is a rabbit polyclonal antibody detecting BRAF in ELISA, IHC, WB. Suitable for Coturnix japonica, Chicken, Human, and Mouse.
Highlights
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| 종 반응성 | Human, Mouse | ||||||||
| 응용 분야 | ELISA, IHC, WB | ||||||||
| 숙주 종 | Rabbit | ||||||||
| 클론성 | Polyclonal | ||||||||
| 이소타입 | IgG | ||||||||
| 면역원 | E. coli - derived recombinant Human BRAF (Asn500-His766). | ||||||||
| 타겟 | BRAF1, RAFB1, Proto-oncogene B-Raf, BRAF, v-Raf murine sarcoma viral oncogene homolog B1, p94, Serine/threonine-protein kinase B-raf | ||||||||
| 정제 | Purified by antigen affinity column. | ||||||||
| 등록번호 | P15056 | ||||||||
| 형태 | Liquid | ||||||||
| 저장 완충액 | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
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| 제품 사용 정보 |
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| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 배경 | Serine/threonine-protein kinase B-raf (BRAF) is a ~84 kDa protein. Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus (Probable). Phosphorylates MAP2K1, and thereby activates the MAP kinase signal transduction pathway. Phosphorylates PFKFB2. May play a role in the postsynaptic responses of hippocampal neurons. 1. Brennan, DF. et al. (2011) Nature 472, 366-9. PMID: 21441910 2. Lavoie, H. et al. (2018) Nature 554, 549-553. PMID: 29433126 3. McGrail, K. et al. (2022) Nature communications 13, 7113. PMID: 36402789 4. Stephens, RM. et al. (1992) Molecular and cellular biology 12, 3733-42. PMID: 1508179 | ||||||||
| 참고사항 | For research use only. |
Western blot analysis was performed using anti-BRAF polyclonal antibody at 1ug/mL on various samples.
Lane 1: K562 cell lysate
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