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Anti-CD10/MME Polyclonal Antibody (HY401014)

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개요
카탈로그 번호HY401014
설명
Anti-CD10/MME Polyclonal Antibody (HY401014) is a rabbit polyclonal antibody detecting CD10 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Pongo abelii, and Pig.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
종 반응성Human, Mouse, Rat
응용 분야ELISA, IHC, WB
숙주 종Rabbit
클론성Polyclonal
이소타입IgG
면역원 E. coli - derived recombinant Human CD10/MME (Met1-Cys80).
타겟 Atriopeptidase, Common acute lymphocytic leukemia antigen, Neutral endopeptidase, Skin fibroblast elastase, CD10, SFE, CALLA, Neprilysin, Neutral endopeptidase 24.11, MME, NEP, Enkephalinase, EPN
정제 Purified by antigen affinity column.
등록번호 P08473
형태 Liquid
저장 완충액 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보
응용 분야 희석
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
안정성 및 저장 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
배경

Neprilysin (CD10/MME) is a ~85 kDa protein. Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Catalyzes cleavage of bradykinin, substance P and neurotensin peptides. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF) and brain natriuretic factor (BNP(1-32)).

1. Rice, GI. et al. (2004) The Biochemical journal 383, 45-51. PMID: 15283675
2. Skidgel, RA. et al. (1984) Peptides 5, 769-76. PMID: 6208535
3. Gafford, JT. et al. (1983) Biochemistry 22, 3265-71. PMID: 6349683
4. Le Moual, H. et al. (1994) European journal of biochemistry 221, 475-80. PMID: 8168535
5. Wisner, A. et al. (2006) Proceedings of the National Academy of Sciences of the United States of America 103, 17979-84. PMID: 17101991
6. Brandt, I. et al. (2006) Clinical chemistry 52, 82-7. PMID: 16254193
참고사항 For research use only.
이미지
참고 문헌
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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