Anti-CKMT1A Polyclonal Antibody (HY409014)

Name: Anti-CKMT1A Polyclonal Antibody
Brand: abinScience
SKU: HY409014
Price: 96 USD
Availability: InStock

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개요
이미지
참고 문헌
데이터시트
SDS

개요

카탈로그 번호

HY409014

설명

Anti-CKMT1A Polyclonal Antibody (HY409014) is a rabbit polyclonal antibody detecting CKMT1A in ELISA, IHC, WB. Suitable for Bovine, Chicken, Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

종 반응성

Human, Mouse, Rat

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human CKMT1A (Ser41-Ile250).

타겟

U-MtCK, CKMT1A, CKMT, Acidic-type mitochondrial creatine kinase, Ubiquitous mitochondrial creatine kinase, Mia-CK, Creatine kinase U-type, mitochondrial

정제

Purified by antigen affinity column.

등록번호

P12532

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Creatine kinase U-type, mitochondrial (CKMT1A) is a ~47 kDa protein. Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.

참고사항

For research use only.

이미지

Immunofluorescence

Hela cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting CKMT1A (HY409014) at a concentration of 12.4 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of CKMT1A was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).

참고 문헌