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| 카탈로그 번호 | JN876013 | ||||||
|---|---|---|---|---|---|---|---|
| 설명 |
Anti-Clostridioides difficile Toxin B/tcdB Nanobody (SAA1173) [SAA1173] (JN876013) is a alpaca monoclonal antibody detecting Clostridium difficile Toxin in ELISA, SPR, WB.
Highlights
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| 종 반응성 | Clostridioides difficile | ||||||
| 응용 분야 | ELISA, SPR, WB | ||||||
| 숙주 종 | Alpaca | ||||||
| 클론성 | Monoclonal | ||||||
| 이소타입 | VHH-8His-Cys-tag | ||||||
| 클론 ID | SAA1173 | ||||||
| 타겟 | Toxin B, 3.4.22.-, Glucosyltransferase TcdB, 2.4.1.-, tcdB, toxB, Clostridium difficile Toxin | ||||||
| 내독소 수준 | Please contact with the lab for this information. | ||||||
| 순도 | >95% as determined by SDS-PAGE. | ||||||
| 정제 | Purified by Nickel column. | ||||||
| 등록번호 | P18177 | ||||||
| 형태 | Liquid | ||||||
| 저장 완충액 | 0.01M PBS pH 7.4 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
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| 제품 사용 정보 |
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| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||
| 배경 | Toxin B (tcdB) is a ~269 kDa protein. Precursor of a cytotoxin that targets and disrupts the colonic epithelium, inducing the host inflammatory and innate immune responses and resulting in diarrhea and pseudomembranous colitis. TcdB constitutes the main toxin that mediates the pathology of C.difficile infection, an opportunistic pathogen that colonizes the colon when the normal gut microbiome is disrupted. Compared to TcdA, TcdB is more virulent and more important for inducing the host inflammatory and innate immune responses. This form constitutes the precursor of the toxin: it enters into host cells and mediates autoprocessing to release the active toxin (Glucosyltransferase TcdB) into the host cytosol. Targets colonic epithelia by binding to the frizzled receptors FZD1, FZD2 and FZD7, and enters host cells via clathrin-mediated endocytosis. 1. Kuehne, SA. et al. (2010) Nature 467, 711-3. PMID: 20844489 2. Xu, H. et al. (2014) Nature 513, 237-41. PMID: 24919149 3. Lyras, D. et al. (2009) Nature 458, 1176-9. PMID: 19252482 4. Qa'Dan, M. et al. (2000) Infection and immunity 68, 2470-4. PMID: 10768933 5. Barth, H. et al. (2001) The Journal of biological chemistry 276, 10670-6. PMID: 11152463 6. Pfeifer, G. et al. (2003) The Journal of biological chemistry 278, 44535-41. PMID: 12941936 7. Reineke, J. et al. (2007) Nature 446, 415-9. PMID: 17334356 9. Tao, L. et al. (2016) Nature 538, 350-355. PMID: 27680706 | ||||||
| 참고사항 | For research use only. |
SDS-PAGE for Anti-Clostridioides difficile Toxin B/tcdB Nanobody (SAA1173)
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