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Anti-Clostridium perfringens pfo/Perfringolysin O/Theta-toxin Recombinant Antibody (HS2) (JN963013)

Anti-Clostridium perfringens pfo/Perfringolysin O/Theta-toxin Recombinant Antibody (HS2)
Anti-Clostridium perfringens pfo/Perfringolysin O/Theta-toxin Recombinant Antibody (HS2)
Anti-Clostridium perfringens pfo/Perfringolysin O/Theta-toxin Recombinant Antibody (HS2)
Anti-Clostridium perfringens pfo/Perfringolysin O/Theta-toxin Recombinant Antibody (HS2)
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사양:
  • 20ug
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  • 100ug
  • 1mg
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  • 개요

  • 이미지

  • 참고 문헌

  • 데이터시트

  • SDS

개요
카탈로그 번호JN963013
종 반응성Clostridium perfringens
응용 분야ELISA, Neutralization, WB
숙주 종Mouse
클론성Monoclonal
이소타입IgG2a
클론 IDHS2
타겟 Perfringolysin O, PFO, Theta-toxin, Thiol-activated cytolysin, pfo, pfoA, pfoR, CPE0163, Clostridium perfringens (strain 13 / Type A)
내독소 수준 Please contact with the lab for this information.
순도 >95% as determined by SDS-PAGE.
정제 Protein A/G purified from cell culture supernatant.
등록번호 P0C2E9
RRID

Anti-Clostridium perfringens pfo/Perfringolysin O/Theta-toxin Antibody (HS2) (abinScience Cat# JN963013, RRID:AB_3729017)
_x005F_x005F_x000D_

형태 Liquid
저장 완충액 0.01M PBS pH 7.4

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보
응용 분야 희석
ELISA 1:2000-1:20000
WB 1:500-1:2000
안정성 및 저장 Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
배경

Perfringolysin O (pfo/Theta-toxin) is a ~55 kDa protein. A cholesterol-dependent toxin that causes cytolysis by forming pores in cholesterol-containing host membranes. After binding to target membranes, the protein assembles into a pre-pore complex. A major conformational change leads to insertion in the host membrane and formation of an oligomeric pore complex. Cholesterol is required for binding to host cell membranes, membrane insertion and pore formation; cholesterol binding is mediated by a Thr-Leu pair in the C-terminus. Can be reversibly inactivated by oxidation.

참고사항 For research use only.
이미지
  • Anti-Clostridium perfringens pfo/Perfringolysin O/Theta-toxin Recombinant Antibody (HS2)

    SDS-PAGE

    SDS-PAGE for Anti-Clostridium perfringens pfo/Perfringolysin O/Theta-toxin Antibody (HS2)

  • Anti-Clostridium perfringens pfo/Perfringolysin O/Theta-toxin Recombinant Antibody (HS2)

    Bioactivity

    Detects recombinant Clostridium perfringens pfo/Perfringolysin O/Theta-toxin (Catalog No: JN963012) in indirect ELISA

참고 문헌
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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