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| 카탈로그 번호 | YP539203 | ||||
|---|---|---|---|---|---|
| 응용 분야 | DB, ELISA, IF | ||||
| 숙주 종 | Mouse | ||||
| 클론성 | Monoclonal | ||||
| 이소타입 | IgG2a, kappa | ||||
| 클론 ID | 3E10# | ||||
| 타겟 | DNA, dsDNA | ||||
| 내독소 수준 | Please contact with the lab for this information. | ||||
| 순도 | >95% as determined by SDS-PAGE. | ||||
| 정제 | Protein A/G purified from cell culture supernatant. | ||||
| RRID | Anti-dsDNA Antibody (3E10#) (abinScience Cat# YP539203, RRID:AB_3725561) |
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| 형태 | Liquid | ||||
| 저장 완충액 | 0.94 mM Citric acid monohydrate, 16.7mM Trisodium citrate dihydrate, 6.2% sucrose, pH 6.5, 0.01% Tween 80 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
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| 제품 사용 정보 |
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| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. | ||||
| 참고사항 | For research use only. |
SDS-PAGE for Anti-dsDNA Antibody (3E10#).
Hela cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting dsDNA (YP539203) overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Mouse IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of dsDNA was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).
MG63 cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting dsDNA (YP539203) overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Mouse IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of dsDNA was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).
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