Anti-E2F1/RBBP3 Polyclonal Antibody (HX082014)

Name: Anti-E2F1/RBBP3 Polyclonal Antibody
Brand: abinScience
SKU: HX082014
Price: 168 USD
Availability: InStock

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100ug

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개요
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참고 문헌
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SDS

개요

카탈로그 번호

HX082014

설명

Anti-E2F1/RBBP3 Polyclonal Antibody (HX082014) is a rabbit polyclonal antibody detecting E2F1/RBBP3 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

종 반응성

Human

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human E2F1/RBBP3 (Val88-Phe437).

타겟

Retinoblastoma-binding protein 3, Retinoblastoma-associated protein 1, RBBP-3, Transcription factor E2F1, RBBP3, E2F1, E2F-1, RBAP-1, pRB-binding protein E2F-1, PBR3

정제

Purified by antigen affinity column.

등록번호

Q01094

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Transcription factor E2F1 (RBBP3) is a ~46 kDa protein. Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can mediate both cell proliferation and TP53/p53-dependent apoptosis. Blocks adipocyte differentiation by binding to specific promoters repressing CEBPA binding to its target gene promoters.

1. Martínez-Balbás, MA. et al. (2000) The EMBO journal 19, 662-71. PMID: 10675335

2. Stevens, C. et al. (2003) Nature cell biology 5, 401-9. PMID: 12717439

3. García-Alvarez, G. et al. (2007) Biochimica et biophysica acta 1773, 375-82. PMID: 17050006

4. Wang, C. et al. (2007) The Journal of biological chemistry 282, 29902-9. PMID: 17704056

5. Wang, C. et al. (2008) FEBS letters 582, 2793-8. PMID: 18625225

6. Wang, D. et al. (2018) Journal of molecular cell biology 10, 60-73. PMID: 28992046

8. Zaragoza, K. et al. (2010) Molecular and cellular biology 30, 2293-304. PMID: 20176812

참고사항

For research use only.

이미지

참고 문헌

데이터시트

Anti-E2F1/RBBP3 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-E2F1/RBBP3 Polyclonal Antibody (HX082014)

개요

이미지

참고 문헌

데이터시트

SDS