Anti-ERCC5 Polyclonal Antibody (HB668014)

Name: Anti-ERCC5 Polyclonal Antibody
Brand: abinScience
SKU: HB668014
Price: 168 USD
Availability: InStock

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개요
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SDS

개요

카탈로그 번호

HB668014

설명

Anti-ERCC5 Polyclonal Antibody (HB668014) is a rabbit polyclonal antibody detecting ERCC5 in ELISA, IHC, WB. Suitable for Human and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

종 반응성

Human

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human ERCC5 (Leu778-Ala986).

타겟

ERCC5, DNA excision repair protein ERCC-5, ERCM2, XPGC, Xeroderma pigmentosum group G-complementing protein, XPG, DNA repair protein complementing XP-G cells

정제

Purified by antigen affinity column.

등록번호

P28715

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

DNA excision repair protein ERCC-5 (ERCC5) is a ~133 kDa protein. Single-stranded structure-specific DNA endonuclease involved in DNA excision repair. Makes the 3'incision in DNA nucleotide excision repair (NER). Binds and bends DNA repair bubble substrate and breaks base stacking at the single-strand/double-strand DNA junction of the DNA bubble. Plays a role in base excision repair (BER) by promoting the binding of DNA glycosylase NTHL1 to its substrate and increasing NTHL1 catalytic activity that removes oxidized pyrimidines from DNA. Involved in transcription-coupled nucleotide excision repair (TCR) which allows RNA polymerase II-blocking lesions to be rapidly removed from the transcribed strand of active genes.

1. Tsutakawa, SE. et al. (2020) Proceedings of the National Academy of Sciences of the United States of America 117, 14127-14138. PMID: 32522879

2. González-Corrochano, R. et al. (2020) Nucleic acids research 48, 9943-9958. PMID: 32821917

3. Cloud, KG. et al. (1995) Mutation research 347, 55-60. PMID: 7651464

4. Habraken, Y. et al. (1994) Nucleic acids research 22, 3312-6. PMID: 8078765

5. O'Donovan, A. et al. (1994) Nature 371, 432-5. PMID: 8090225

6. O'Donovan, A. et al. (1994) The Journal of biological chemistry 269, 15965-8. PMID: 8206890

7. Bessho, T. (1999) Nucleic acids research 27, 979-83. PMID: 9927729

8. Sarker, AH. et al. (2005) Molecular cell 20, 187-98. PMID: 16246722

10. Kokic, G. et al. (2019) Nature communications 10, 2885. PMID: 31253769

참고사항

For research use only.

이미지

참고 문헌

데이터시트

Anti-ERCC5 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-ERCC5 Polyclonal Antibody (HB668014)

개요

이미지

참고 문헌

데이터시트

SDS