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Anti-HBoV1 Minor capsid protein VP1 Polyclonal Antibody (VK140014)

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개요
카탈로그 번호VK140014
설명
Anti-HBoV1 Minor capsid protein VP1 Polyclonal Antibody (VK140014) is a rabbit polyclonal antibody detecting Minor capsid protein VP1. Suitable for Primate bocaparvovirus 1.
종 반응성Primate bocaparvovirus 1 (Human bocavirus 1 type 1) (HBoV1)
응용 분야ELISA, IHC, WB
숙주 종Rabbit
클론성Polyclonal
이소타입IgG
면역원 E. coli - derived recombinant HBoV1 Minor capsid protein VP1 (Lys181-Gly290).
타겟 EC:3.1.1.4, Minor capsid protein VP1, VP1
내독소 수준 Please contact with the lab for this information.
정제 Purified by antigen affinity column.
등록번호 Q3YPH4
형태 Liquid
저장 완충액 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

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제품 사용 정보
응용 분야 희석
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
배경

Minor capsid protein VP1 is a ~75 kDa protein. Capsid proteins self-assembles to form an icosahedral capsid with a T=1 symmetry, about 26 nm in diameter, and consisting of 60 copies of three size variants of the capsid proteins, VP1, and VP3, which differ by the presence of an N-terminal extension in the minor protein VP1 (Probable). The capsid has a channel at the 5-fold axis and there are densities extending the 5-fold axis into the interior of the capsid. The capsid encapsulates the genomic ssDNA (Probable). Binding to the host receptors also induces capsid rearrangements leading to surface exposure of VP1 N-terminus, specifically its phospholipase A2-like region. The additional N-terminal region of isoform Minor capsid protein VP1, called VP1u, may serve as a lipolytic enzyme to breach the endosomal membrane during entry into host cell and might contribute to virus transport to the nucleus.

1. Mietzsch, M. et al. (2017) Journal of virology 91. PMID: 28331084
참고사항 For research use only.
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참고 문헌
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Concentration
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Volume
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Formula
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Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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