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Anti-Human ASB9 Polyclonal Antibody (HP570014)

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개요
카탈로그 번호HP570014
종 반응성Human
응용 분야ELISA, IHC, WB
숙주 종Rabbit
클론성Polyclonal
이소타입IgG
면역원 E. coli - derived recombinant Human ASB9 (Ser38-Leu294).
타겟 ASB9, Ankyrin repeat and SOCS box protein 9, ASB-9
정제 Purified by antigen affinity column.
등록번호 Q96DX5
형태 Liquid
저장 완충액 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보
응용 분야 희석
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
안정성 및 저장 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
배경

Ankyrin repeat and SOCS box protein 9 (ASB9) is a ~31 kDa protein. Substrate-recognition component of a cullin-5-RING E3 ubiquitin-protein ligase complex (ECS complex, also named CRL5 complex), which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. The ECS(ASB9) complex catalyzes ubiquitination of creatine kinases CKB and CKMT1A.

1. Balasubramaniam, D. et al. (2015) Biochemistry 54, 1673-80. PMID: 25654263
2. Lumpkin, RJ. et al. (2021) Molecular & cellular proteomics : MCP 20, 100019. PMID: 33268465
3. Kwon, S. et al. (2010) BMC biology 8, 23. PMID: 20302626
4. Fei, X. et al. (2012) The protein journal 31, 275-84. PMID: 22418839
참고사항 For research use only.
이미지
참고 문헌
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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