Human ASB9 Recombinant Protein (N-His) (HP570012)

Name: Human ASB9 Recombinant Protein (N-His)
Brand: abinScience
SKU: HP570012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HP570012
Description	Recombinant Human ASB9 Protein, N-His (HP570012) expressed in E. coli, spanning Ser38-Leu294. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q96DX5
Protein length	Ser38-Leu294
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	30.30 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	ASB9, Ankyrin repeat and SOCS box protein 9, ASB-9
Background	Ankyrin repeat and SOCS box protein 9 (ASB9) is a ~31 kDa protein. Substrate-recognition component of a cullin-5-RING E3 ubiquitin-protein ligase complex (ECS complex, also named CRL5 complex), which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. The ECS(ASB9) complex catalyzes ubiquitination of creatine kinases CKB and CKMT1A. 1. Balasubramaniam, D. et al. (2015) Biochemistry 54, 1673-80. PMID: 25654263 2. Lumpkin, RJ. et al. (2021) Molecular & cellular proteomics : MCP 20, 100019. PMID: 33268465 3. Kwon, S. et al. (2010) BMC biology 8, 23. PMID: 20302626 4. Fei, X. et al. (2012) The protein journal 31, 275-84. PMID: 22418839
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human ASB9 Protein, N-His

References

HP570012-SDS-PAGE-1.jpg

Datasheet

Human ASB9 Recombinant Protein (N-His).pdf

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Formula

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Human ASB9 Recombinant Protein (N-His) (HP570012)

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