Anti-Human ASB9 Polyclonal Antibody (HP570014)

Name: Anti-Human ASB9 Polyclonal Antibody
Brand: abinScience
SKU: HP570014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HP570014

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human ASB9 (Ser38-Leu294).

Target

ASB9, Ankyrin repeat and SOCS box protein 9, ASB-9

Purification

Purified by antigen affinity column.

Accession

Q96DX5

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Ankyrin repeat and SOCS box protein 9 (ASB9) is a ~31 kDa protein. Substrate-recognition component of a cullin-5-RING E3 ubiquitin-protein ligase complex (ECS complex, also named CRL5 complex), which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. The ECS(ASB9) complex catalyzes ubiquitination of creatine kinases CKB and CKMT1A.

1. Balasubramaniam, D. et al. (2015) Biochemistry 54, 1673-80. PMID: 25654263

2. Lumpkin, RJ. et al. (2021) Molecular & cellular proteomics : MCP 20, 100019. PMID: 33268465

3. Kwon, S. et al. (2010) BMC biology 8, 23. PMID: 20302626

4. Fei, X. et al. (2012) The protein journal 31, 275-84. PMID: 22418839

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Human ASB9 polyclonal antibody at 1ug/mL on recombinant Human ASB9 (Catalog No. HP570012).

References

Datasheet

Anti-Human ASB9 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human ASB9 Polyclonal Antibody (HP570014)

Overview

Images

References

Datasheet

SDS

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