Anti-Human ASF1A Polyclonal Antibody (HP534014)

Name: Anti-Human ASF1A Polyclonal Antibody
Brand: abinScience
SKU: HP534014
Price: 168 USD
Availability: InStock

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100ug

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개요
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참고 문헌
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SDS

개요

카탈로그 번호

HP534014

설명

Anti-Human ASF1A Polyclonal Antibody (HP534014) is a rabbit polyclonal antibody detecting ASF1A in ELISA, IHC, WB. Suitable for Human, Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

종 반응성

Human, Mouse

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human ASF1A (Met1-His150).

타겟

hAsf1, CCG1-interacting factor A, hAsf1a, Anti-silencing function protein 1 homolog A, Histone chaperone ASF1A, CIA, ASF1A, hCIA

정제

Purified by antigen affinity column.

등록번호

Q9Y294

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Histone chaperone ASF1A is a ~22 kDa protein. Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly and with HIRA to promote replication-independent chromatin assembly. Promotes homologous recombination-mediated repair of double-strand breaks (DSBs) at stalled or collapsed replication forks: acts by mediating histone replacement at DSBs, leading to recruitment of the MMS22L-TONSL complex and subsequent loading of RAD51. Also involved in the nuclear import of the histone H3-H4 dimer together with importin-4 (IPO4): specifically recognizes and binds newly synthesized histones with the monomethylation of H3 'Lys-9' and acetylation at 'Lys-14' (H3K9me1K14ac) marks, and diacetylation at 'Lys-5' and 'Lys-12' of H4 (H4K5K12ac) marks in the cytosol. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit.

1. Munakata, T. et al. (2000) Genes to cells : devoted to molecular & cellular mechanisms 5, 221-33. PMID: 10759893

2. Mello, JA. et al. (2002) EMBO reports 3, 329-34. PMID: 11897662

3. Umehara, T. et al. (2003) The Journal of biological chemistry 278, 35660-7. PMID: 12842904

4. Tagami, H. et al. (2004) Cell 116, 51-61. PMID: 14718166

5. Groth, A. et al. (2005) Molecular cell 17, 301-11. PMID: 15664198

6. Tamburini, BA. et al. (2005) Eukaryotic cell 4, 1583-90. PMID: 16151251

7. Alvarez, F. et al. (2011) The Journal of biological chemistry 286, 17714-21. PMID: 21454524

10. Zhang, R. et al. (2005) Developmental cell 8, 19-30. PMID: 15621527

참고사항

For research use only.

이미지

참고 문헌

데이터시트

Anti-Human ASF1A Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-Human ASF1A Polyclonal Antibody (HP534014)

개요

이미지

참고 문헌

데이터시트

SDS