Human ASF1A Recombinant Protein (N-His) (HP534012)

Name: Human ASF1A Recombinant Protein (N-His)
Brand: abinScience
SKU: HP534012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HP534012
Description	Recombinant Human ASF1A Protein, N-His (HP534012) expressed in E. coli, spanning Met1-His150. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q9Y294
Protein length	Met1-His150
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	19.18 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	hAsf1, CCG1-interacting factor A, hAsf1a, Anti-silencing function protein 1 homolog A, Histone chaperone ASF1A, CIA, ASF1A, hCIA
Background	Histone chaperone ASF1A is a ~22 kDa protein. Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly and with HIRA to promote replication-independent chromatin assembly. Promotes homologous recombination-mediated repair of double-strand breaks (DSBs) at stalled or collapsed replication forks: acts by mediating histone replacement at DSBs, leading to recruitment of the MMS22L-TONSL complex and subsequent loading of RAD51. Also involved in the nuclear import of the histone H3-H4 dimer together with importin-4 (IPO4): specifically recognizes and binds newly synthesized histones with the monomethylation of H3 'Lys-9' and acetylation at 'Lys-14' (H3K9me1K14ac) marks, and diacetylation at 'Lys-5' and 'Lys-12' of H4 (H4K5K12ac) marks in the cytosol. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit. 1. Munakata, T. et al. (2000) Genes to cells : devoted to molecular & cellular mechanisms 5, 221-33. PMID: 10759893 2. Mello, JA. et al. (2002) EMBO reports 3, 329-34. PMID: 11897662 3. Umehara, T. et al. (2003) The Journal of biological chemistry 278, 35660-7. PMID: 12842904 4. Tagami, H. et al. (2004) Cell 116, 51-61. PMID: 14718166 5. Groth, A. et al. (2005) Molecular cell 17, 301-11. PMID: 15664198 6. Tamburini, BA. et al. (2005) Eukaryotic cell 4, 1583-90. PMID: 16151251 7. Alvarez, F. et al. (2011) The Journal of biological chemistry 286, 17714-21. PMID: 21454524 10. Zhang, R. et al. (2005) Developmental cell 8, 19-30. PMID: 15621527
Note	For research use only.

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SDS-PAGE for Recombinant Human ASF1A Protein, N-His

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Datasheet

Human ASF1A Recombinant Protein (N-His).pdf

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Human ASF1A Recombinant Protein (N-His) (HP534012)

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