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Anti-Human ATP5PB Monoclonal Antibody (1A481) (HC151025)

Anti-Human ATP5PB Monoclonal Antibody (1A481)
Anti-Human ATP5PB Monoclonal Antibody (1A481)
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개요
카탈로그 번호HC151025
설명
Anti-Human ATP5PB Monoclonal Antibody (1A481) [1A481] (HC151025) is a mouse monoclonal antibody detecting ATP5PB in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Recombinant — Superior batch-to-batch reproducibility with defined sequences.
  • ●Multi-Application — Validated across multiple applications.
종 반응성Human
응용 분야ELISA, IHC, WB
숙주 종Mouse
클론성Monoclonal
이소타입IgG
클론 ID1A481
면역원 E. coli - derived recombinant human ATP5PB (Ser96-Met256).
타겟 ATP synthase peripheral stalk subunit b mitochondrial, ATP synthase F(0) complex subunit B1 mitochondrial, ATP synthase peripheral stalk-membrane subunit b, ATP synthase proton-transporting mitochondrial F(0) complex subunit B1, ATP synthase subunit b, ATPase subunit b, ATP5PB
내독소 수준 Please contact with the lab for this information.
순도 >95% as determined by SDS-PAGE.
정제 Protein A/G purified from cell culture supernatant.
등록번호 P24539
형태 Liquid
저장 완충액 0.01M PBS pH 7.4.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

안정성 및 저장 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
배경

ATP synthase peripheral stalk subunit b, mitochondrial (ATP5PB) is a ~28 kDa protein. Subunit b, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. ATP synthase complex consist of a soluble F(1) head domain - the catalytic core - and a membrane F(1) domain - the membrane proton channel. These two domains are linked by a central stalk rotating inside the F(1) region and a stationary peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation (Probable). In vivo, can only synthesize ATP although its ATP hydrolase activity can be activated artificially in vitro.

1. Lai, Y. et al. (2023) Molecular cell 83, 2137-2147.e4. PMID: 37244256
참고사항 For research use only.
이미지
  • Anti-Human ATP5PB Monoclonal Antibody (1A481)

    SDS-PAGE

    SDS-PAGE for Anti-Human ATP5PB Monoclonal Antibody (1A481)

참고 문헌
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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