中文
English
한국어
日本語
Español
Français
Русский
| 카탈로그 번호 | HX250013 | ||||||
|---|---|---|---|---|---|---|---|
| 종 반응성 | Human | ||||||
| 응용 분야 | ELISA, FCM, WB | ||||||
| 숙주 종 | Mouse | ||||||
| 클론성 | Monoclonal | ||||||
| 이소타입 | IgG2b-kappa | ||||||
| 클론 ID | BBM.1 | ||||||
| 타겟 | Beta-2-microglobulin, Beta-2-microglobulin form pI 5.3, B2M, CDABP0092, HDCMA22P | ||||||
| 내독소 수준 | Please contact with the lab for this information. | ||||||
| 순도 | >95% as determined by SDS-PAGE. | ||||||
| 정제 | Protein A/G purified from cell culture supernatant. | ||||||
| 등록번호 | P61769 | ||||||
| 형태 | Liquid | ||||||
| 저장 완충액 | 0.01M PBS pH 7.4 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
||||||
| 제품 사용 정보 |
| ||||||
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. | ||||||
| 참고사항 | For research use only. | ||||||
| Isotype Control | Mouse IgG2b, kappa Recombinant Isotype Control Antibody (MPC-11) (MF974207) |
SEC-HPLC detection for Anti-Human B2M/Beta-2-microglobulin Antibody (BBM.1).
Detects B2M/Beta-2-microglobulin in indirect ELISAs.
Flow-cytometry using anti-human B2M antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human B2M antibody monoclonal antibody (Catalog # HX250013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry using anti-human B2M antibody.Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human B2M antibody monoclonal antibody (Catalog # HX250013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry using anti-human B2M antibody.A431 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human B2M antibody monoclonal antibody (Catalog # HX250013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry using anti-human B2M antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human B2M antibody monoclonal antibody (Catalog # HX250013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry using anti-human B2M antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human B2M antibody monoclonal antibody (Catalog # HX250013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE for Anti-Human B2M/Beta-2-microglobulin Antibody (BBM.1).
Flow-cytometry using APC anti-human B2M antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human B2M monoclonal antibody (Catalog: HX250013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry using FITC anti-human B2M antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human B2M monoclonal antibody (Catalog: HX250013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry using PE anti-human B2M antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human B2M monoclonal antibody (Catalog: HX250013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Western blot analysis was performed using anti-CD1a & B2M monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human CD1a & B2M (Catalog No: HY043011)
Lane 2: negative control
+86-027-65523339
중국 우한시 심둔사로 666번지 C동, 우한, 430206
