Anti-Human IL-6R alpha/IL6R Recombinant Antibody (SAA0048) (HY583107)

Name: Anti-Human IL-6R alpha/IL6R Recombinant Antibody (SAA0048)
Brand: abinScience
SKU: HY583107
Price: 191 USD
Availability: InStock

데이터시트 제품 공유 정보 복사

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개요
이미지
참고 문헌
데이터시트
SDS

개요

카탈로그 번호	HY583107
종 반응성	Human
응용 분야	ELISA, FCM, IHC, WB
숙주 종	Mouse
클론성	Monoclonal
이소타입	IgG1, kappa
클론 ID	SAA0048
테스트된 응용 분야	WB, FCM
타겟	CD126, IL-6 receptor subunit alpha, IL-6R 1, IL-6R subunit alpha, IL-6R-alpha, IL-6RA, IL6R, Interleukin-6 receptor subunit alpha, Membrane glycoprotein 80, Soluble interleukin-6 receptor subunit alpha, gp80, sIL6R
내독소 수준	< 10 EU/mg
순도	>95% as determined by SDS-PAGE.
정제	Protein A/G purified from cell culture supernatant.
등록번호	P08887
형태	Liquid
저장 완충액	0.01M PBS pH 7.4. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.
안정성 및 저장	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
배경	Interleukin-6 receptor subunit alpha (CD126/IL6R) is a ~51 kDa protein. Part of the receptor for interleukin 6. Binds to IL6 with low affinity, but does not transduce a signal. Signal activation necessitate an association with IL6ST. Activation leads to the regulation of the immune response, acute-phase reactions and hematopoiesis. The interaction with membrane-bound IL6R and IL6ST stimulates 'classic signaling', the restricted expression of the IL6R limits classic IL6 signaling to only a few tissues such as the liver and some cells of the immune system. 1. Larsen, JV. et al. (2017) Molecular and cellular biology 37. PMID: 28265003 2. Kang, S. et al. (2019) Immunity 50, 1007-1023. PMID: 30995492 3. Spencer, S. et al. (2019) The Journal of experimental medicine 216, 1986-1998. PMID: 31235509
참고사항	For research use only.

이미지

Flow Cytometry

Flow-cytometry using anti-human CD126 antibody.CD126 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD126 antibody monoclonal antibody (Catalog # HY583107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human CD126 antibody.U937 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD126 antibody monoclonal antibody (Catalog # HY583107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using anti-human CD126 antibody. U937 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD126 monoclonal antibody (Catalog: HY583107, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Western blot

Western blot analysis was performed using anti-CD126/IL6RA monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human CD126/IL6RA (Catalog No: HY583011)
Lane 2: negative control