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Anti-Human DEP-1/CD148 Polyclonal Antibody (HC363014)

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개요
카탈로그 번호HC363014
설명
Anti-Human DEP-1/CD148 Polyclonal Antibody (HC363014) is a rabbit polyclonal antibody detecting DEP-1/CD148 in ELISA, IHC, WB. Suitable for Human, Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
종 반응성Human, Mouse
응용 분야ELISA, IHC, WB
숙주 종Rabbit
클론성Polyclonal
이소타입IgG
면역원 E. coli - derived recombinant Human CD148/PTPRJ (Arg997-Ala1337).
타겟 HPTP eta, R-PTP-J, Protein-tyrosine phosphatase eta, Density-enhanced phosphatase 1, Receptor-type tyrosine-protein phosphatase eta, Protein-tyrosine phosphatase receptor type J, DEP-1, R-PTP-eta, CD148, PTPRJ, DEP1
내독소 수준 Please contact with the lab for this information.
순도 >95% as determined by SDS-PAGE.
정제 Purified by antigen affinity column.
등록번호 Q12913
형태 Liquid
저장 완충액 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보
응용 분야 희석
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
안정성 및 저장 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
배경

Receptor-type tyrosine-protein phosphatase eta (CD148/PTPRJ) is a ~145 kDa protein. Tyrosine phosphatase which dephosphorylates or contributes to the dephosphorylation of CTNND1, FLT3, PDGFRB, MET, KDR, LYN, SRC, MAPK1, MAPK3, EGFR, TJP1, OCLN, PIK3R1 and PIK3R2. Plays a role in cell adhesion, migration, proliferation and differentiation. Has a role in megakaryocytes and platelet formation. Involved in vascular development. Regulator of macrophage adhesion and spreading.

1. Kovalenko, M. et al. (2000) The Journal of biological chemistry 275, 16219-26. PMID: 10821867
2. Persson, C. et al. (2002) FEBS letters 517, 27-31. PMID: 12062403
3. Holsinger, LJ. et al. (2002) Oncogene 21, 7067-76. PMID: 12370829
4. Palka, HL. et al. (2003) The Journal of biological chemistry 278, 5728-35. PMID: 12475979
5. Tsuboi, N. et al. (2008) The Biochemical journal 413, 193-200. PMID: 18348712
6. Sacco, F. et al. (2009) The Journal of biological chemistry 284, 22048-22058. PMID: 19494114
7. Omerovic, J. et al. (2010) The Biochemical journal 426, 65-72. PMID: 19922411
8. Arora, D. et al. (2011) The Journal of biological chemistry 286, 10918-29. PMID: 21262971
9. Kellie, S. et al. (2004) Journal of cell science 117, 609-18. PMID: 14709717
참고사항 For research use only.
이미지
참고 문헌
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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