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Anti-Human Fc gamma RIIIA/CD16a2/SELPLG Recombinant Antibody (SAA0065) (HC298107)

Anti-Human Fc gamma RIIIA/CD16a2/SELPLG Recombinant Antibody (SAA0065)
Anti-Human Fc gamma RIIIA/CD16a2/SELPLG Recombinant Antibody (SAA0065)
Anti-Human Fc gamma RIIIA/CD16a2/SELPLG Recombinant Antibody (SAA0065)
Anti-Human Fc gamma RIIIA/CD16a2/SELPLG Recombinant Antibody (SAA0065)
Anti-Human Fc gamma RIIIA/CD16a2/SELPLG Recombinant Antibody (SAA0065)
Anti-Human Fc gamma RIIIA/CD16a2/SELPLG Recombinant Antibody (SAA0065)
Anti-Human Fc gamma RIIIA/CD16a2/SELPLG Recombinant Antibody (SAA0065)
Anti-Human Fc gamma RIIIA/CD16a2/SELPLG Recombinant Antibody (SAA0065)
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  • 개요

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  • 데이터시트

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개요
카탈로그 번호HC298107
종 반응성Human
응용 분야ELISA, FCM
숙주 종Human
클론성Monoclonal
이소타입IgG1, kappa
클론 IDSAA0065
타겟 Selectin P ligand, PSGL-1, SELPLG, CD162, P-selectin glycoprotein ligand 1
내독소 수준 Please contact with the lab for this information.
순도 >95% as determined by SDS-PAGE.
정제 Protein A/G purified from cell culture supernatant.
등록번호 Q14242
형태 Liquid
저장 완충액 0.01M PBS pH 7.4

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

안정성 및 저장 Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
배경

P-selectin glycoprotein ligand 1 (CD162/SELPLG) is a ~43 kDa protein. A SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. Critical for the initial leukocyte capture.

참고사항 For research use only.
이미지
  • Anti-Human Fc gamma RIIIA/CD16a2/SELPLG Recombinant Antibody (SAA0065)

    Bioactivity

    SEC-HPLC detection for Anti-Human CD162/SELPLG Antibody (ABS0199).

  • Anti-Human Fc gamma RIIIA/CD16a2/SELPLG Recombinant Antibody (SAA0065)

    Flow Cytometry

    Flow-cytometry using anti-human CD162 antibody.Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD162 antibody monoclonal antibody (Catalog # HC298107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.

  • Anti-Human Fc gamma RIIIA/CD16a2/SELPLG Recombinant Antibody (SAA0065)

    Flow Cytometry

    Flow-cytometry using anti-human CD162 antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD162 antibody monoclonal antibody (Catalog # HC298107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.

  • Anti-Human Fc gamma RIIIA/CD16a2/SELPLG Recombinant Antibody (SAA0065)

    Flow Cytometry

    Flow-cytometry using anti-human CD162 antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD162 antibody monoclonal antibody (Catalog # HC298107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.

참고 문헌
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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