Anti-Human Fc gamma RIIIA/CD16a Recombinant Antibody (SAA2437) (HY386033)

Name: Anti-Human Fc gamma RIIIA/CD16a Recombinant Antibody (SAA2437)
Brand: abinScience
SKU: HY386033
Price: 126 USD
Availability: InStock

데이터시트 제품 공유 정보 복사

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사양:

20ug
50ug
100ug
1mg

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개요
이미지
참고 문헌
데이터시트
SDS

개요

카탈로그 번호

HY386033

종 반응성

Human

응용 분야

ELISA, FACS, Functional assay

숙주 종

Mouse

클론성

Monoclonal

이소타입

IgG2a, lambda

클론 ID

SAA2437

테스트된 응용 분야

FCM

타겟

Low affinity immunoglobulin gamma Fc region receptor III-A, IgG Fc receptor III-A, CD16-II, CD16a antigen, Fc-gamma RIII-alpha, Fc-gamma RIII, Fc-gamma RIIIa, FcRIII, FcRIIIa, FcgammaRIIIA, FcR-10, IgG Fc receptor III-2, CD16a, FCGR3A, CD16A, FCG3, FCGR3, IGFR3

내독소 수준

Please contact with the lab for this information.

순도

>95% as determined by SDS-PAGE.

정제

Protein A/G purified from cell culture supernatant.

등록번호

P08637

형태

Liquid

저장 완충액

0.01M PBS pH 7.4

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:2000-1:20000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Low affinity immunoglobulin gamma Fc region receptor III-A (CD16a) is a ~29 kDa protein. Receptor for the invariable Fc fragment of immunoglobulin gamma (IgG). Optimally activated upon binding of clustered antigen-IgG complexes displayed on cell surfaces, triggers lysis of antibody-coated cells, a process known as antibody-dependent cellular cytotoxicity (ADCC). Does not bind free monomeric IgG, thus avoiding inappropriate effector cell activation in the absence of antigenic trigger. Mediates IgG effector functions on natural killer (NK) cells. Binds antigen-IgG complexes generated upon infection and triggers NK cell-dependent cytokine production and degranulation to limit viral load and propagation.

1. Hezareh, M. et al. (2001) Journal of virology 75, 12161-8. PMID: 11711607

2. Ferrara, C. et al. (2011) Proceedings of the National Academy of Sciences of the United States of America 108, 12669-74. PMID: 21768335

3. Mizushima, T. et al. (2011) Genes to cells : devoted to molecular & cellular mechanisms 16, 1071-80. PMID: 22023369

4. DiLillo, DJ. et al. (2014) Nature medicine 20, 143-51. PMID: 24412922

5. Lee, J. et al. (2015) Immunity 42, 431-42. PMID: 25786175

6. Jing, Y. et al. (2015) PloS one 10, e0121788. PMID: 25816339

7. Blázquez-Moreno, A. et al. (2017) Proceedings of the National Academy of Sciences of the United States of America 114, E5645-E5654. PMID: 28652325

8. de Haas, M. et al. (1996) Journal of immunology (Baltimore, Md. : 1950) 156, 2948-55. PMID: 8609432

9. Koene, HR. et al. (1997) Blood 90, 1109-14. PMID: 9242542

참고사항

For research use only

Isotype Control

Mouse IgG2a (Fc Silence) Recombinant Isotype Control (MOPC 173) (MF533023)

이미지

SDS-PAGE

SDS-PAGE for Anti-Human CD16a/FCGR3A Antibody (SAA2437)
Flow cytometry

Flow-cytometry APC using anti-human CD16A antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an APC anti-human CD16A monoclonal antibody (Catalog HY386033) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow cytometry

Flow-cytometry PE using anti-human CD16A antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an PE anti-human CD16A monoclonal antibody (Catalog HY386033) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow cytometry

Flow-cytometry FITC using anti-human CD16A antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an FITC anti-human CD16A monoclonal antibody (Catalog HY386033) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.