Anti-Human DNAM-1/CD226 Polyclonal Antibody (HS737014)

Name: Anti-Human DNAM-1/CD226 Polyclonal Antibody
Brand: abinScience
SKU: HS737014
Price: 96 USD
Availability: InStock

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개요
이미지
참고 문헌
데이터시트
SDS

개요

카탈로그 번호

HS737014

설명

Anti-Human CD226/DNAM-1 Polyclonal Antibody (HS737014) is a rabbit polyclonal antibody detecting DNAM-1 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

종 반응성

Human

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human CD226/DNAM-1 (Glu19-Glu242).

타겟

CD226 antigen, DNAX accessory molecule 1, DNAM-1, CD226, CD226, DNAM1

정제

Purified by antigen affinity column.

등록번호

Q15762

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

CD226 antigen (DNAM-1) is a ~38 kDa protein. Cell surface receptor that plays an important role in the immune system, particularly in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-cells and NK cells. Functions as a costimulatory receptor upon recognition of target cells, such as virus-infected or tumor cells. Upon binding to its ligands PVR/CD155 or NECTIN2/CD112 on target cells, promotes the cytotoxic activity of NK cells and CTLs, enhancing their ability to kill these cells. Mechanistically, phosphorylation by Src kinases such as LYN of FYN, enables binding to adapter GRB2, leading to activation of VAV1, PI3K and PLCG1. Promotes also activation of kinases ERK and AKT, as well as calcium fluxes.

1. Shibuya, A. et al. (1996) Immunity 4, 573-81. PMID: 8673704

2. Shibuya, A. et al. (1998) Journal of immunology (Baltimore, Md. : 1950) 161, 1671-6. PMID: 9712030

3. Zhu, Y. et al. (2016) The Journal of experimental medicine 213, 167-76. PMID: 26755705

4. Deuss, FA. et al. (2019) The Journal of biological chemistry 294, 12534-12546. PMID: 31253644

5. Wang, H. et al. (2019) Proceedings of the National Academy of Sciences of the United States of America 116, 988-996. PMID: 30591568

참고사항

For research use only.

이미지

Western blot

Western blot analysis was performed using anti-CD226 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Jurkat cell lysate
Lane 2: PC-12 cell lysate
Lane 3: Hela cell lysate
Lane 4: Mouse spleen lysate
Lane 5: Mouse lung lysate
Lane 6: Rat spleen lysate
Lane 7: Rat lung lysate