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Anti-Human CD24 Recombinant Antibody (SAA2212), APC (HB630337)

Anti-Human CD24 Recombinant Antibody (SAA2212), APC
Anti-Human CD24 Recombinant Antibody (SAA2212), APC
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  • 개요

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개요
카탈로그 번호HB630337
종 반응성Human
응용 분야ELISA, FCM
숙주 종Human
클론성Monoclonal
이소타입IgG1, kappa
클론 IDSAA2212
접합APC
테스트된 응용 분야 FCM
타겟 Signal transducer CD24, CD24, CD24A, Small cell lung carcinoma cluster 4 antigen
내독소 수준 Please contact with the lab for this information.
관련 제품 Human IgG1 Recombinant Isotype Control Antibody (HyHEL-10), APC
정제 Affinity chromatography.
등록번호 P25063
형태 Liquid
저장 완충액 0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

안정성 및 저장 Store at 4°C for 12 months. Protect from light. Do not freeze.
배경

Signal transducer CD24 is a ~8 kDa protein. May have a pivotal role in cell differentiation of different cell types. Signaling could be triggered by the binding of a lectin-like ligand to the CD24 carbohydrates, and transduced by the release of second messengers derived from the GPI-anchor. Modulates B-cell activation responses. Promotes AG-dependent proliferation of B-cells, and prevents their terminal differentiation into antibody-forming cells. In association with SIGLEC10 may be involved in the selective suppression of the immune response to danger-associated molecular patterns (DAMPs) such as HMGB1, HSP70 and HSP90.

1. Suzuki, T. et al. (2001) Journal of immunology (Baltimore, Md. : 1950) 166, 5567-77. PMID: 11313396
참고사항 For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.
이미지
  • Anti-Human CD24 Recombinant Antibody (SAA2212), APC

    Flow Cytometry

    Flow-cytometry using APC anti-Human CD24 antibody. SK-BR-3 cells were stained with an irrelevant antibody (Green Histogram) or an APC anti-Human CD24 antibody monoclonal antibody (Catalog # HB630337 , Red Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

참고 문헌
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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