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Anti-Human CEACAM-8/CD66b Recombinant Antibody (BW250/183) (HB202107)

Anti-Human CEACAM-8/CD66b Recombinant Antibody (BW250/183)
Anti-Human CEACAM-8/CD66b Recombinant Antibody (BW250/183)
Anti-Human CEACAM-8/CD66b Recombinant Antibody (BW250/183)
Anti-Human CEACAM-8/CD66b Recombinant Antibody (BW250/183)
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  • 개요

  • 이미지

  • 참고 문헌

  • 데이터시트

  • SDS

개요
카탈로그 번호HB202107
종 반응성Human
응용 분야FCM
숙주 종Mouse
클론성Monoclonal
이소타입IgG1, kappa
클론 IDBW250/183
타겟 CD67 antigen, Carcinoembryonic antigen CGM6, Carcinoembryonic antigen-related cell adhesion molecule 8, CGM6, CD66b, Non-specific cross-reacting antigen NCA-95, CEACAM8
내독소 수준 Please contact with the lab for this information.
순도 >95% as determined by SDS-PAGE.
정제 Protein A/G purified from cell culture supernatant.
등록번호 P31997
형태 Liquid
저장 완충액 0.01M PBS pH 7.4

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

안정성 및 저장 Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
배경

Cell adhesion molecule CEACAM8 (CD66b) is a ~38 kDa protein. Cell surface glycoprotein that plays a role in cell adhesion in a calcium-independent manner. Mediates heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM6. Heterophilic interaction with CEACAM8 occurs in activated neutrophils.

1. Kuroki, M. et al. (2001) Journal of leukocyte biology 70, 543-50. PMID: 11590190
2. Oikawa, S. et al. (1991) The Journal of biological chemistry 266, 7995-8001. PMID: 2022629
3. Yamanaka, T. et al. (1996) Protein expression and purification 7, 438-46. PMID: 8776764
참고사항 For research use only.
이미지
  • Anti-Human CEACAM-8/CD66b Recombinant Antibody (BW250/183)

    Bioactivity

    SEC-HPLC detection for Anti-Human CD66b/CEACAM8 Antibody (BW250/183).

  • Anti-Human CEACAM-8/CD66b Recombinant Antibody (BW250/183)

    Flow CytoMetry

    Flow-cytometry using anti-human CD66b antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD66b monoclonal antibody (Catalog: HB202107) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.

참고 문헌
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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