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Anti-Human CD9 Recombinant Antibody (SAA0003), PE (HB984127)

Anti-Human CD9 Recombinant Antibody (SAA0003), PE
Anti-Human CD9 Recombinant Antibody (SAA0003), PE
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사양:
  • 50T
  • 100T
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  • 개요

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개요
카탈로그 번호HB984127
종 반응성Human
응용 분야ELISA, FCM, WB
숙주 종Human
클론성Monoclonal
이소타입IgG1
클론 IDSAA0003
접합PE
테스트된 응용 분야 FCM
타겟 Motility-related protein, Leukocyte antigen MIC3, TSPAN29, Cell growth-inhibiting gene 2 protein, CD9, MIC3, p24, Tspan-29, MRP-1, 5H9 antigen, Tetraspanin-29, CD9 antigen, RHD48801
내독소 수준 Please contact with the lab for this information.
관련 제품 Human IgG1 Recombinant Isotype Control Antibody (HyHEL-10), PE
등록번호 P21926
형태 Liquid
저장 완충액 0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

안정성 및 저장 Store at 4°C for 12 months. Protect from light. Do not freeze.
배경

CD9 antigen is a ~25 kDa protein. Integral membrane protein associated with integrins, which regulates different processes, such as sperm-egg fusion, platelet activation and aggregation, and cell adhesion. Present at the cell surface of oocytes and plays a key role in sperm-egg fusion, possibly by organizing multiprotein complexes and the morphology of the membrane required for the fusion. In myoblasts, associates with CD81 and PTGFRN and inhibits myotube fusion during muscle regeneration. In macrophages, associates with CD81 and beta-1 and beta-2 integrins, and prevents macrophage fusion into multinucleated giant cells specialized in ingesting complement-opsonized large particles. Also prevents the fusion between mononuclear cell progenitors into osteoclasts in charge of bone resorption.

1. Higginbottom, A. et al. (2003) Biochemical and biophysical research communications 311, 208-14. PMID: 14575715
2. Nakazawa, Y. et al. (2008) Blood 112, 1730-9. PMID: 18541721
3. Ikeyama, S. et al. (1993) The Journal of experimental medicine 177, 1231-7. PMID: 8478605
4. Takeda, Y. et al. (2003) The Journal of cell biology 161, 945-56. PMID: 12796480
5. Masellis-Smith, A. et al. (1994) Journal of immunology (Baltimore, Md. : 1950) 152, 2768-77. PMID: 7511626
참고사항 For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.
이미지
  • Anti-Human CD9 Recombinant Antibody (SAA0003), PE

    Flow CytoMetry

    Flow-cytometry using PE anti-human CD9 antibody. HCT-116 cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD9 monoclonal antibody (Catalog: HB984127, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.

참고 문헌
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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