Anti-Human CRBN Polyclonal Antibody (HP395014)

Name: Anti-Human CRBN Polyclonal Antibody
Brand: abinScience
SKU: HP395014
Price: 168 USD
Availability: InStock

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50ug
100ug

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개요
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참고 문헌
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개요

카탈로그 번호

HP395014

설명

Anti-Human CRBN Polyclonal Antibody (HP395014) is a rabbit polyclonal antibody detecting CRBN in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

종 반응성

Human, Mouse, Rat

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human CRBN (Met1-Leu442).

타겟

Protein cereblon, CRBN, AD-006

정제

Purified by antigen affinity column.

등록번호

Q96SW2

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Protein cereblon (CRBN) is a ~50 kDa protein. Substrate recognition component of a DCX (DDB1-CUL4-X-box) E3 protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins, such as MEIS2, ILF2 or GLUL. Normal degradation of key regulatory proteins is required for normal limb outgrowth and expression of the fibroblast growth factor FGF8. Maintains presynaptic glutamate release and consequently cognitive functions, such as memory and learning, by negatively regulating large-conductance calcium-activated potassium (BK) channels in excitatory neurons. Likely to function by regulating the assembly and neuronal surface expression of BK channels via its interaction with KCNT1. May also be involved in regulating anxiety-like behaviors via a BK channel-independent mechanism.

1. Nguyen, TV. et al. (2016) Molecular cell 61, 809-20. PMID: 26990986

2. Lian, Q. et al. (2020) The protein journal 39, 411-421. PMID: 33009960

3. Ito, T. et al. (2010) Science (New York, N.Y.) 327, 1345-50. PMID: 20223979

4. Gandhi, AK. et al. (2014) British journal of haematology 164, 811-21. PMID: 24328678

5. Fischer, ES. et al. (2014) Nature 512, 49-53. PMID: 25043012

6. Chamberlain, PP. et al. (2014) Nature structural & molecular biology 21, 803-9. PMID: 25108355

7. Higgins, JJ. et al. (2008) Neurogenetics 9, 219-23. PMID: 18414909

8. Choi, TY. et al. (2018) The Journal of neuroscience : the official journal of the Society for Neuroscience 38, 3571-3583. PMID: 29530986

9. Kim, MJ. et al. (2019) Frontiers in immunology 10, 2203. PMID: 31620128

참고사항

For research use only.

이미지

참고 문헌

데이터시트

Anti-Human CRBN Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-Human CRBN Polyclonal Antibody (HP395014)

개요

이미지

참고 문헌

데이터시트

SDS