Human CRBN Recombinant Protein (N-His) (HP395012)

Name: Human CRBN Recombinant Protein (N-His)
Brand: abinScience
SKU: HP395012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HP395012
Description	Recombinant Human CRBN Protein, N-His (HP395012) expressed in E. coli, spanning Met1-Leu442. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q96SW2
Protein length	Met1-Leu442
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	52.71 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Protein cereblon, CRBN, AD-006
Background	Protein cereblon (CRBN) is a ~50 kDa protein. Substrate recognition component of a DCX (DDB1-CUL4-X-box) E3 protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins, such as MEIS2, ILF2 or GLUL. Normal degradation of key regulatory proteins is required for normal limb outgrowth and expression of the fibroblast growth factor FGF8. Maintains presynaptic glutamate release and consequently cognitive functions, such as memory and learning, by negatively regulating large-conductance calcium-activated potassium (BK) channels in excitatory neurons. Likely to function by regulating the assembly and neuronal surface expression of BK channels via its interaction with KCNT1. May also be involved in regulating anxiety-like behaviors via a BK channel-independent mechanism. 1. Nguyen, TV. et al. (2016) Molecular cell 61, 809-20. PMID: 26990986 2. Lian, Q. et al. (2020) The protein journal 39, 411-421. PMID: 33009960 3. Ito, T. et al. (2010) Science (New York, N.Y.) 327, 1345-50. PMID: 20223979 4. Gandhi, AK. et al. (2014) British journal of haematology 164, 811-21. PMID: 24328678 5. Fischer, ES. et al. (2014) Nature 512, 49-53. PMID: 25043012 6. Chamberlain, PP. et al. (2014) Nature structural & molecular biology 21, 803-9. PMID: 25108355 7. Higgins, JJ. et al. (2008) Neurogenetics 9, 219-23. PMID: 18414909 8. Choi, TY. et al. (2018) The Journal of neuroscience : the official journal of the Society for Neuroscience 38, 3571-3583. PMID: 29530986 9. Kim, MJ. et al. (2019) Frontiers in immunology 10, 2203. PMID: 31620128
Note	For research use only.

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SDS-PAGE for Recombinant Human CRBN Protein, N-His

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Datasheet

Human CRBN Recombinant Protein (N-His).pdf

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Human CRBN Recombinant Protein (N-His) (HP395012)

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