Anti-Human CUL1 Polyclonal Antibody (HC489014)

Name: Anti-Human CUL1 Polyclonal Antibody
Brand: abinScience
SKU: HC489014
Price: 96 USD
Availability: InStock

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개요
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참고 문헌
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SDS

개요

카탈로그 번호

HC489014

설명

Anti-Human CUL1 Polyclonal Antibody (HC489014) is a rabbit polyclonal antibody detecting CUL1 in ELISA, IHC, WB. Suitable for Mus musculus and Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

종 반응성

Human, Mouse

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human CUL1 (Met1-Glu250).

타겟

CUL1

정제

Purified by antigen affinity column.

등록번호

Q13616

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

대체 이름

Cullin-1, CUL-1, CUL1

배경

Cullin-1 (CUL1) is a ~89 kDa protein. Core component of multiple cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. SCF complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins. In the SCF complex, serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. May contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and exchange of the substrate recognition component is mediated by TIP120A/CAND1.

1. Gao, D. et al. (2011) Molecular cell 44, 290-303. PMID: 22017875

2. Duan, S. et al. (2011) Molecular cell 44, 317-24. PMID: 22017877

3. Scott, DC. et al. (2016) Cell 166, 1198-1214.e24. PMID: 27565346

4. Liwocha, J. et al. (2024) Nature structural & molecular biology 31, 378-389. PMID: 38326650

5. Min, KW. et al. (2003) The Journal of biological chemistry 278, 15905-10. PMID: 12609982

6. Pagan, JK. et al. (2015) Nature cell biology 17, 31-43. PMID: 25503564

7. Man, X. et al. (2015) European journal of cell biology 94, 162-72. PMID: 25704143

8. Hopf, LVM. et al. (2022) Nature structural & molecular biology 29, 854-862. PMID: 35982156

10. Muhar, MF. et al. (2025) Nature 638, 519-527. PMID: 39880951

참고사항

For research use only.

이미지

Western blot

Western blot analysis was performed using anti-CUL1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Mouse brain lysate
Lane 2: Rat brain lysate

참고 문헌

데이터시트

Anti-Human CUL1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-Human CUL1 Polyclonal Antibody (HC489014)

개요

이미지

참고 문헌

데이터시트

SDS