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Anti-Human DPEP3 Recombinant Antibody (B43), APC (HV426137)

Anti-Human DPEP3 Recombinant Antibody (B43), APC
Anti-Human DPEP3 Recombinant Antibody (B43), APC
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  • 개요

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  • 데이터시트

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개요
카탈로그 번호HV426137
종 반응성Human
응용 분야ELISA, FCM
숙주 종Mouse
클론성Monoclonal
이소타입IgG2a, kappa
클론 IDB43
접합APC
타겟 DPEP3, Dipeptidase 3
내독소 수준 Please contact with the lab for this information.
관련 제품 Mouse IgG2a, kappa Recombinant Isotype Control Antibody (MOPC-173), APC
정제 Affinity chromatography.
등록번호 Q9H4B8
형태 Liquid
저장 완충액 0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

안정성 및 저장 Store at 4°C for 12 months. Protect from light. Do not freeze.
배경

Dipeptidase 3 (DPEP3) is a ~53 kDa protein. Lacks dipeptidase activity and is unable to hydrolyze cystinyl-bis-glycine, leukotriene D4 and the beta-lactam antibiotic imipenem. The absence of activity may be due to the inability of asparagine (instead of aspartate found in DPEP1/2) at position 359 to function as the acid/base catalyst and activate the nucleophilic water/hydroxide. A tyrosine (instead of histidine) at position 269 reduces affinity for the beta zinc and may cause substrate steric hindrance.

1. Hayashi, K. et al. (2020) Journal of structural biology 211, 107512. PMID: 32325220
참고사항 For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.
이미지
  • Anti-Human DPEP3 Recombinant Antibody (B43), APC

    Flow Cytometry

    Flow-cytometry using APC anti-Human CD19 antibody. Raji cells were stained with an irrelevant antibody (Green Histogram) or an APC anti-Human CD19 antibody monoclonal antibody (Catalog # HV426137 , Red Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

참고 문헌
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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