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Anti-Human Coagulation Factor IX Recombinant Antibody (SAA2440) (HF916023)

Anti-Human Coagulation Factor IX Recombinant Antibody (SAA2440)
Anti-Human Coagulation Factor IX Recombinant Antibody (SAA2440)
Anti-Human Coagulation Factor IX Recombinant Antibody (SAA2440)
Anti-Human Coagulation Factor IX Recombinant Antibody (SAA2440)
가격(USD):
사양:
  • 20ug
  • 50ug
  • 100ug
  • 1mg
수량:
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  • 개요

  • 이미지

  • 참고 문헌

  • 데이터시트

  • SDS

개요
카탈로그 번호HF916023
설명
Anti-Human Coagulation Factor IX Recombinant Antibody (SAA2440) (HF916023) is a human monoclonal antibody detecting Coagulation Factor IX in ELISA, Bioactivity: FCM, Functional assay, Research in vivo. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
종 반응성Human
응용 분야ELISA, Bioactivity: FCM, Functional assay, Research in vivo
숙주 종Human
클론성Monoclonal
이소타입IgG4, kappa
클론 IDSAA2440
타겟 Coagulation factor IX, EC:3.4.21.22, Christmas factor, Plasma thromboplastin component, PTC, Coagulation factor IXa light chain, Coagulation factor IXa heavy chain, F9
내독소 수준 < 10 EU/mg
순도 >95% as determined by SDS-PAGE.
정제 Protein A/G purified from cell culture supernatant.
등록번호 P00740
형태 Liquid
저장 완충액 0.01M PBS pH 7.4.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보
응용 분야 희석
ELISA 1:2000-1:20000
안정성 및 저장 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
배경

Coagulation factor IX (F9) is a ~51 kDa protein. Factor IX is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca(2+) ions, phospholipids, and factor VIIIa.

1. de la Salle, C. et al. (1993) Nouvelle revue francaise d'hematologie 35, 473-80. PMID: 8295821
2. Suehiro, K. et al. (1989) The Journal of biological chemistry 264, 21257-65. PMID: 2592373
3. Wang, S. et al. (2010) Journal of medicinal chemistry 53, 1473-82. PMID: 20121197
4. Wang, S. et al. (2010) Journal of medicinal chemistry 53, 1465-72. PMID: 20121198
5. Rawala-Sheikh, R. et al. (1992) Blood 79, 398-405. PMID: 1730085
6. Simioni, P. et al. (2009) The New England journal of medicine 361, 1671-5. PMID: 19846852
7. Cao, Q. et al. (2025) Nature 639, 816-824. PMID: 39880037
참고사항 For research use only
Isotype Control Human IgG4 (S228P) Recombinant Isotype Control Antibody (13R4) (HP298207)
이미지
  • Anti-Human Coagulation Factor IX Recombinant Antibody (SAA2440)

    SDS-PAGE

    SDS-PAGE for Anti-Human F9/Factor IX Antibody (SAA2440)

  • Anti-Human Coagulation Factor IX Recombinant Antibody (SAA2440)

    Flow cytometry

    Flow-cytometry using anti-human F9 antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human F9 monoclonal antibody (Catalog HF916023, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.

참고 문헌
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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