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| 카탈로그 번호 | HY030045 |
|---|---|
| 설명 |
Anti-Human GAPDH Monoclonal Antibody (1A201) [1A201] (HY030045) is a mouse monoclonal antibody detecting GAPDH in ELISA, WB. Suitable for Human, Mouse, and Rat.
Highlights
|
| 종 반응성 | Human, Mouse, Rat |
| 응용 분야 | ELISA, WB |
| 숙주 종 | Mouse |
| 클론성 | Monoclonal |
| 이소타입 | IgG2b |
| 클론 ID | 1A201 |
| 면역원 | E. coli - derived recombinant Human GAPDH protein (Met1-Glu335). |
| 타겟 | GAPD, Peptidyl-cysteine S-nitrosylase GAPDH, GAPDH, Glyceraldehyde-3-phosphate dehydrogenase |
| 내독소 수준 | < 10 EU/mg |
| 순도 | >95% as determined by SDS-PAGE. |
| 정제 | Protein A/G purified from cell culture supernatant. |
| 형태 | Liquid |
| 저장 완충액 | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 배경 | Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a ~36 kDa protein. Catalyzes the conversion of D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate in glycolysis and the reverse reaction in gluconeogenesis. Also shows nitrosylase activity, thereby playing a role in nuclear functions. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. 1. Tisdale, EJ. (2002) The Journal of biological chemistry 277, 3334-41. PMID: 11724794 2. Ercolani, L. et al. (1988) The Journal of biological chemistry 263, 15335-41. PMID: 3170585 3. Arif, A. et al. (2012) Molecular and cellular biology 32, 5046-55. PMID: 23071094 4. Gao, X. et al. (2013) Cell host & microbe 13, 87-99. PMID: 23332158 5. Gao, X. et al. (2016) The Journal of biological chemistry 291, 18232-8. PMID: 27387501 |
| 참고사항 | For research use only. |
SDS-PAGE for Anti-Human GAPDH Monoclonal Antibody (1A201)
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