Anti-Human Granzyme B Recombinant Antibody (Fv02), FITC (HY574117)

Name: Anti-Human Granzyme B Recombinant Antibody (Fv02), FITC
Brand: abinScience
SKU: HY574117
Price: 223 USD
Availability: InStock

데이터시트 제품 공유 정보 복사

가격(USD):

사양:

50T
100T

수량:

문의하기

개요
이미지
참고 문헌
데이터시트
SDS

개요

카탈로그 번호	HY574117
종 반응성	Human
응용 분야	FCM
숙주 종	Mouse
클론성	Monoclonal
이소타입	IgG1
클론 ID	Fv02
접합	FITC
타겟	Cathepsin G-like 1, Granzyme-2, GZMB, grzmB, Fragmentin-2, Human lymphocyte protein, HLP, CTLA-1, CGL1, CTSGL1, CSPB, Lymphocyte protease, T-cell serine protease 1-3E, Granzyme B, Granzyme-B, CTLA1, GRB, C11, Cytotoxic T-lymphocyte proteinase 2, SECT
내독소 수준	Please contact with the lab for this information.
관련 제품	Mouse IgG1 Recombinant Isotype Control Antibody (HyHEL-10), FITC
등록번호	P10144
형태	Liquid
저장 완충액	0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.
안정성 및 저장	Store at 4°C for 12 months. Protect from light. Do not freeze.
배경	Granzyme B (GZMB) is a ~27 kDa protein. Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent pyroptosis when delivered into the target cell through the immunological synapse. It cleaves after Asp. Once delivered into the target cell, acts by catalyzing cleavage of gasdermin-E (GSDME), releasing the pore-forming moiety of GSDME, thereby triggering pyroptosis and target cell death. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -9 and -10 (CASP3, CASP9 and CASP10, respectively) to give rise to active enzymes mediating apoptosis. 1. Poe, M. et al. (1991) The Journal of biological chemistry 266, 98-103. PMID: 1985927 2. Hameed, A. et al. (1988) Journal of immunology (Baltimore, Md. : 1950) 141, 3142-7. PMID: 3262682 3. Krähenbühl, O. et al. (1988) Journal of immunology (Baltimore, Md. : 1950) 141, 3471-7. PMID: 3263427 4. Froelich, CJ. et al. (1993) Journal of immunology (Baltimore, Md. : 1950) 151, 7161-71. PMID: 8258716 5. Liu, Y. et al. (2020) Science immunology 5. PMID: 31953257 6. Zhang, Z. et al. (2020) Nature 579, 415-420. PMID: 32188940 7. Yang, X. et al. (1998) The Journal of biological chemistry 273, 34278-83. PMID: 9852092
참고사항	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

이미지

Flow Cytometry

Flow-cytometry using FITC anti-Human GZMB antibody. BXPC3 cells were stained with an irrelevant antibody (Green Histogram) or a FITC anti-Human GZMB antibody monoclonal antibody (Catalog # HY574117 , Red Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

참고 문헌

데이터시트