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| 카탈로그 번호 | HY574013 | ||||||
|---|---|---|---|---|---|---|---|
| 종 반응성 | Human | ||||||
| 응용 분야 | ELISA, WB | ||||||
| 숙주 종 | Mouse | ||||||
| 클론성 | Monoclonal | ||||||
| 이소타입 | IgG1 | ||||||
| 클론 ID | SAA0515 | ||||||
| 타겟 | Granzyme-B, Cathepsin G-like 1, Granzyme-2, grzmB, GZMB, Fragmentin-2, Human lymphocyte protein, HLP, CTLA-1, CGL1, CTSGL1, CSPB, Lymphocyte protease, T-cell serine protease 1-3E, Granzyme B, CTLA1, GRB, C11, Cytotoxic T-lymphocyte proteinase 2, SECT | ||||||
| 내독소 수준 | Please contact with the lab for this information. | ||||||
| 순도 | >95% as determined by SDS-PAGE. | ||||||
| 정제 | Protein A/G purified from cell culture supernatant. | ||||||
| 등록번호 | P10144 | ||||||
| RRID | Anti-Human GZMB Antibody (SAA0515) (abinScience Cat# HY574013, RRID:AB_3725204) |
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| 형태 | Liquid | ||||||
| 저장 완충액 | 0.01M PBS pH 7.4 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
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| 제품 사용 정보 |
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| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. | ||||||
| 배경 | Granzyme B (GZMB) is a ~27 kDa protein. Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent pyroptosis when delivered into the target cell through the immunological synapse. It cleaves after Asp. Once delivered into the target cell, acts by catalyzing cleavage of gasdermin-E (GSDME), releasing the pore-forming moiety of GSDME, thereby triggering pyroptosis and target cell death. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -9 and -10 (CASP3, CASP9 and CASP10, respectively) to give rise to active enzymes mediating apoptosis. 1. Poe, M. et al. (1991) The Journal of biological chemistry 266, 98-103. PMID: 1985927 2. Hameed, A. et al. (1988) Journal of immunology (Baltimore, Md. : 1950) 141, 3142-7. PMID: 3262682 3. Krähenbühl, O. et al. (1988) Journal of immunology (Baltimore, Md. : 1950) 141, 3471-7. PMID: 3263427 4. Froelich, CJ. et al. (1993) Journal of immunology (Baltimore, Md. : 1950) 151, 7161-71. PMID: 8258716 5. Liu, Y. et al. (2020) Science immunology 5. PMID: 31953257 6. Zhang, Z. et al. (2020) Nature 579, 415-420. PMID: 32188940 7. Yang, X. et al. (1998) The Journal of biological chemistry 273, 34278-83. PMID: 9852092 | ||||||
| 참고사항 | For research use only. | ||||||
| Isotype Control | Mouse IgG1, kappa Recombinant Isotype Control Antibody (MOPC-21) (MV080207) |
SEC-HPLC detection for Anti-Human GZMB Antibody (ABS0372).
SDS-PAGE for Anti-Human GZMB Antibody (SAA0515).
Western blot analysis was performed using anti-GZMB monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human GZMB (Catalog No: HY574012)
Lane 2: negative control
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