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Anti-Human Jo-1/HARS1 Recombinant Antibody (SAA2345) (HY584023)

Anti-Human Jo-1/HARS1 Recombinant Antibody (SAA2345)
Anti-Human Jo-1/HARS1 Recombinant Antibody (SAA2345)
가격(USD):
사양:
  • 20ug
  • 50ug
  • 100ug
  • 1mg
수량:
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  • 개요

  • 이미지

  • 참고 문헌

  • 데이터시트

  • SDS

개요
카탈로그 번호HY584023
종 반응성Human
응용 분야ELISA, WB
숙주 종Human
클론성Monoclonal
이소타입IgG1
클론 IDSAA2345
타겟 Histidine--tRNA ligase cytoplasmic, EC:6.1.1.21, Histidyl-tRNA synthetase, HisRS, HARS1, HARS, HRS, jo-1
내독소 수준 < 10 EU/mg
순도 >95% as determined by SDS-PAGE.
정제 Protein A/G purified from cell culture supernatant.
등록번호 P12081
형태 Liquid
저장 완충액 0.01M PBS pH 7.4.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보
응용 분야 희석
ELISA 1:2000-1:20000
WB 1:500-1:2000
안정성 및 저장 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
배경

Histidine--tRNA ligase, cytoplasmic (HARS1/jo-1) is a ~57 kDa protein. Catalyzes the ATP-dependent ligation of histidine to the 3'-end of its cognate tRNA, via the formation of an aminoacyl-adenylate intermediate (His-AMP). Plays a role in axon guidance.

1. Abbott, JA. et al. (2018) Human mutation 39, 415-432. PMID: 29235198
2. Safka Brozkova, D. et al. (2015) Brain : a journal of neurology 138, 2161-72. PMID: 26072516
참고사항 For research use only
이미지
  • Anti-Human Jo-1/HARS1 Recombinant Antibody (SAA2345)

    Western blot

    Western blot analysis was performed using anti-HARS1 monoclonal antibody at 1ug/mL on various samples.
    Lane 1: Hela cell lysate
    Lane 2: 293T cell lysate
    Lane 3: K562 cell lysate
    Lane 4: HepG2 cell lysate
    Lane 5: HCT116 cell lysate
    Lane 6: Jurkat cell lysate

참고 문헌
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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